▎ 摘　　要

NOVELTY - Detecting neonicotinoid pesticide residue in water using graphene, by (a) adding graphene to water sample, adjusting pH and vortexing/oscillating solution so that neonicotinoid pesticide residue gets absorbed on the graphene, (b) centrifuging the water sample and discarding aqueous layer, (c) adding extracting solvent to precipitate, (d) concentrating by adding sodium chloride to precipitate, centrifuging solution, separating and spin evaporating upper layer, drying solution and adding solvent to solution, (e) detecting sample, and (f) recovering the graphene. USE - The method is useful for detecting neonicotinoid pesticide residue in water. ADVANTAGE - The used graphene can be recovered up to 80-110%, using organic solvent and distilled water after processing the water sample and the graphene can be reused again. The method enables highly accurate and efficient detection of the neonicotinoid pesticide residue in water sample, as the graphene is used as absorption material which is required in lesser amount and has strong absorbing ability and low preparation cost. The method is convenient, rapid and easy to operate, when compared to the existing methodologies. The method avoids performing repeated extraction and usage of solid phase extraction (SPE) columns. The method is environmentally-friendly and cost-effective. DETAILED DESCRIPTION - Method for detecting neonicotinoid pesticide residue in water using graphene, involves (a) adding 5-30 mg of graphene to 10-50 ml of collected water sample containing neonicotinoid pesticide residue, adjusting pH of obtained solution, and vortexing or oscillating the solution for few seconds so that target substance i.e. neonicotinoid pesticide residue gets absorbed on the graphene, (b) subjecting the neonicotinoid pesticide residue containing water sample to centrifugation and discarding aqueous layer to obtain precipitate, (c) adding 4-8 ml of extracting solvent to the precipitate for extracting the neonicotinoid pesticide from the precipitate, (d) concentrating by adding sodium chloride to the precipitate, subjecting obtained solution to centrifugation, separating upper layer of the solution, subjecting separated upper layer of solution to spin evaporation, drying solution using nitrogen, and adding 1 ml of solvent to dried product to obtain sample having constant volume, (e) detecting predetermined volume of the sample by detection instrument, and (f) washing the used graphene sequentially using an organic solvent and distilled water for recovering the graphene.