▎ 摘　　要

NOVELTY - Amplifying dendritic cells (DC) from peripheral blood involves (a) separating mononuclear cells from peripheral blood, and separating monocytes by magnetic bead sorting; (b) differentiating the monocytes separated by magnetic beads into DC cells; (c) transferring the differentiated DC cells to culture medium for culturing, where base of culture medium is made of graphene medium with conductive properties, and graphene base is connected to the micro-current delivery device; and (d) adding first-stage expansion medium synchronously after transferring the DC cells into the culture medium, culturing it for 2-4 hours, and observing the changes of cell maturation, and when activity of the cells is enhanced then second-stage induction solution is replaced, and micro-current delivery is carried out to graphene base through the micro-current delivery device, and DC cells are directly stimulated by the micro-current. USE - Method for amplifying dendritic cells from peripheral blood. ADVANTAGE - The method amplifies dendritic cells from peripheral blood, which divides expansion process of DC cells into three stages, changes the base of the traditional medium, stimulates and enhances total protein content, adenosine triphosphate activity and exercise intensity of cells by dual stimulation using the current-assisted culture medium, accelerates growth rate of cell, enhances robustness of cell, shortens culture time for cell expansion and enhances overall activity of mature DC cell. DETAILED DESCRIPTION - Amplifying dendritic cells (DC) from peripheral blood involves (a) separating mononuclear cells from peripheral blood, and separating monocytes by magnetic bead sorting; (b) differentiating the monocytes separated by magnetic beads into DC cells; (c) transferring the differentiated DC cells to culture medium for culturing, where base of culture medium is made of graphene medium with conductive properties, and graphene base is connected to the micro-current delivery device; and (d) adding first-stage expansion medium synchronously after transferring the DC cells into the culture medium, culturing it for 2-4 hours, and observing the changes of cell maturation, and when activity of the cells is enhanced then second-stage induction solution is replaced, and micro-current delivery is carried out to graphene base through the micro-current delivery device, and DC cells are directly stimulated by the micro-current. The total protein amount in DC cells, ATP activity in cells and the movement of cells are enhanced to accelerate the growth rate of DC cells, where third-stage stimulation solution is replaced after 1 day of culture, and then microcurrent is assisted to stimulate growth, where DC cells fully mature after two days of bidirectional stimulation.