• 专利标题:   Single atom cofactor-mimicking carbon nanozyme used in biosensor e.g. immunodiagnostic sensor, or active oxygen scavenger, comprises carbon nanomaterials, and transition metal monoatomic active site coupled to surface of carbon nanomaterial.
  • 专利号:   KR2022071388-A, KR2498026-B1
  • 发明人:   LEEJINWOO, JOONSANG Y, KIM M I, FONTINEUING
  • 专利权人:   KOREA ADVANCED SCI TECHNOLOGY INST, UNIV GACHON IND ACADEMIC COOP FOUND
  • 国际专利分类:   B82Y015/00, B82Y040/00, C01B032/05, C01B032/15, C01B032/198, C12N009/08, C12Q001/30, C12Q001/54, G01N033/543
  • 专利详细信息:   KR2022071388-A 31 May 2022 C01B-032/15 202255 Pages: 17
  • 申请详细信息:   KR2022071388-A KR158428 24 Nov 2020
  • 优先权号:   KR158428

▎ 摘  要

NOVELTY - Single atom cofactor-mimicking carbon nanozyme comprises carbon nanomaterials, and a transition metal monoatomic active site coupled to the surface of the carbon nanomaterial and distributed in a size of atom, where the monoatomic active site of the transition metal has a monoatomic structure in which nitrogen is bonded to a single atom of the transition metal. USE - The single atom cofactor-mimicking carbon nanozyme is useful in biosensor (immunodiagnostic sensor, hydrogen peroxide sensor, or glucose sensor), or active oxygen scavenger (all claimed) for performing immunodiagnosis and processing environmental and biohazardous substances. ADVANTAGE - The single atom cofactor-mimicking carbon nanozyme has excellent stability, high enzyme-mimicking activity and selectivity by mimicking actual structure of a biological protein coenzyme, replaces existing catalase and peroxide removal enzymes, can be produced and purified in a cost-effective manner, and performs immunodiagnosis with high-sensitivity. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is included for a method for producing the single atom cofactor-mimicking carbon nanozyme, which involves dispersing each of the carbon nanomaterial and transition metal precursor in distilled water to prepare a carbon nanomaterial dispersion and a transition metal precursor dispersion, cooling the mixture comprising carbon nanomaterial dispersion and transition metal precursor dispersion, freeze-drying the cooled mixture, and preparing a carbon nanozyme composed of a transition metal monoatomic active site-carbon nanomaterial complex structure by heat-treating the freeze-dried mixture while flowing ammonia gas.