▎ 摘　　要

NOVELTY - An acetylcholinesterase/graphene quantum dot/fluorine-nitrogen-doped titanium dioxide nano-tube array photoelectrochemical biosensor, comprises graphene quantum dot/fluorine-nitrogen-doped titanium dioxide nano-tube array and acetylcholinesterase, where the graphene quantum dot/fluorine-nitrogen-doped titanium dioxide nano-tube array comprises a nitrogen-fluorine-doped titanium dioxide nanotube array and a modification graphene quantum dots on a titanium dioxide nanotube array, and the acetylcholinesterase crosslinks are modified on the graphene quantum dot/fluorine-nitrogen-doped titanium dioxide nano-tube array. USE - Acetylcholinesterase/graphene quantum dot/fluorine-nitrogen-doped titanium dioxide nano-tube array photoelectrochemical biosensor for use in studying inhibitory effect of ketamine on acetylcholinesterase and detecting derivatives of acetylcholine and acetylcholine (all claimed). ADVANTAGE - The acetylcholinesterase/graphene quantum dot/fluorine-nitrogen-doped titanium dioxide nano-tube array photoelectrochemical biosensor has better biological compatibility, improved electrode fixing function, affinity and photo-generated current strength modification. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is also included for a method for preparing acetylcholinesterase/graphene quantum dot/fluorine-nitrogen-doped titanium dioxide nano-tube array photoelectrochemical biosensor, which involves polishing a titanium piece and cleaning with sandpaper of different particle sizes, washing the titanium piece with acetone, absolute ethanol and deionized water, placing the titanium piece in the polishing liquid, carrying out the polishing, after polishing for 1 minute, washing with acetone and deionized water, and drying under a nitrogen atmosphere, utilizing the graphite electrode as a cathode, utilizing the titanium piece as an anode, and applying a voltage of 20 V to contain 0.1 mol/L of ammonium fluoride and 1 mol/L ammonium sulfate is an electrolyte, anodizing for 2 hours, and calcining for 1 hour to obtain an fluorine-nitrogen-doped titanium dioxide nano-tube array electrode, immersing the fluorine-nitrogen-doped titanium dioxide nano-tube array electrode in the solution containing graphene quantum dot on the fluorine-nitrogen-doped titanium dioxide nano-tube array electrode, immersing for 10 minutes, rinsing with double distilled water, and air-drying at room temperature, modifying graphene quantum dot on the surface of the modified electrode of fluorine-nitrogen-doped titanium dioxide nano-tube array to prepare graphene quantum dot/fluorine-nitrogen-doped titanium dioxide nano-tube array electrode, constructing an acetylcholinesterase/graphene quantum dot/fluorine-nitrogen-doped titanium dioxide nano-tube array photoelectrochemical biosensor by utilizing cross-linking method, spraying a mixed solution containing acetylcholinesterase and chitosan onto graphene quantum dot/fluorine-nitrogen-doped titanium dioxide nano-tube array modified electrode and drying to obtain acetylcholinesterase/graphene quantum dot/fluorine-nitrogen-doped titanium dioxide nano-tube array photoelectrochemical biosensor, where the mixed solution of acetylcholinesterase and chitosan had a concentration of acetylcholinesterase of 500 U/mL and a chitosan concentration of 5.0 mg/mL.