▎ 摘　　要

NOVELTY - Dual-functional graphene oxide composite material comprises surface modified amino site graphene oxide raw material including 14-20 wt.% graphene oxide, 14-21 wt.% 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, 32-38 wt.% N-Hydroxysuccinimide and 24-32 wt.% 1,6-Hexanediamine, boric acid grafted graphene oxide including 0.04-0.08 wt.% surface modified amino site graphene oxide, 0.08-0.013 wt.% 2,4-difluoro-3-formylphenylboronic acid, 0.087-0.093 wt.% bovine serum albumin, 88-93 wt.% ethanol, 8.4-9.1 wt.% double distilled water and raw materials loaded with phenolphthalein boron affinity graphene oxide including 0.04-0.08 wt.% boric acid grafted graphene oxide, 0.7-1.4 wt.% phenolphthalein and 92-99 wt.% dimethyl sulfoxide. USE - The material is useful for detecting adherent cells (claimed). ADVANTAGE - The material: has simple, convenient and fast preparation, low cost, good stability and broad application prospects; and can quantitatively detect adherent cells. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are also included for: (1) dual functional graphene oxide composite material comprising (1) dispersing graphene oxide in 10 mM 2-morphine sulfonic acid buffer solution (pH 6) based on measurement, sonicating until the graphene oxide is completely dispersed, adding 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide, shaking slightly at room temperature for 10-15 hours to activate, washing the activated graphene oxide 3 times with 10 mM phosphate buffer (pH 7.4), dispersing in a phosphate buffer, adding 1,6-hexamethylenediamine and shaking at room temperature for 10-15 hours to obtain graphene oxide with modified amino sites on the surface, (2) adding 2,4-difluoro-3-formylphenylboronic acid to the graphene oxide on the surface modified amino site based on measurement, shaking at room temperature for 10-15 hours, wasing with ethanol for 3 times, dispersing in ethanol, adding bovine serum albumin solution dissolved in double-distilled water for inactivation for 2-5 hours, washing 3 times with phosphate buffer solution and dispersing in phosphate buffer solution again to obtain boric acid grafted graphene oxide and (3) dissolving phenolphthalein in dimethyl sulfoxide based on calculated amount, adding to the boric acid grafted graphene oxide obtained in step (2), shaking at room temperature for 10-15 hours, washing 3 times with phosphate buffer, dispersing in phosphate buffer, storing at 4 degrees C and obtaining boron affinity graphene oxide loaded with phenolphthalein, which is bifunctional graphene oxide composite material; and (2) use of dual-functional graphene oxide composite material for detecting adherent cells comprising (1) discarding the original cell culture medium in the cell culture plate and rinsing with 10 mM phosphate buffer (pH 7.4), (2) adding bifunctional graphene oxide composite material and 20 mu g/105 cells based on the mass of graphene oxide, incubating at room temperature for 30 minutes and rinsing with phosphate buffer after completion and (3) adding sodium hydroxide solution to adjust pH 12, after 1 minute of color development, measuring the absorbance value at 553 nm with a microplate reader.