▎ 摘 要
NOVELTY - Functional nano complex comprises 20-40 wt.% biological-enzyme, 32-70 wt.% calcium salt crystals, and 5-30 wt.% graphene oxide. USE - The nano complex is useful in an antimicrobial complex and an adsorption complex. ADVANTAGE - The nano complex: improves activity of immobilized enzymes, thermal stability and storage stability, and has certain antibacterial and adsorption properties. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is also included for preparing the nano complex comprising (i) dispersing the graphene oxide in 0.05-0.2 mol/l phosphate buffer solution and mixing uniformly, (ii) adding the biological enzyme to the mixed solution formed in the step (i), and mixing, (iii) adding the calcium salt solution to the mixture formed in the step (ii), carrying out shaking reaction at 25-30 degrees C under 100-150 revolutions/minute (rpm) for 24-48 hours, and (iv) separating precipitate in the step (iii), and washing and freeze-drying precipitate was at -20 degrees C for 4-6 hours.