▎ 摘　　要

NOVELTY - Biosensor comprises a microfluidics layer, a multimodal layer comprising a counter electrode, a reference electrode, a first working electrode comprising a first detection protein configured to capture a first severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) biomarker. Second working electrode comprises a second detection protein configured to capture a second SARS-CoV-2 biomarker. Logic circuit comprises a processor and a non-transitory memory with computer executable instructions embedded on it. The microfluidics layer comprises multiple microchannels transversely oriented to channel a biological sample from a first surface of the microfluidics layer to a second surface of the microfluidics layer. The biological sample comprises a first and second SARS-CoV-2 biomarker. The multimodal sensing layer is fluidically coupled to the second surface of the microfluidics layer to receive the biological sample from the microchannels. USE - Biosensor for detecting SARS-CoV-2 biological markers. ADVANTAGE - The biosensor provides sensitive, rapid, inexpensive, quantitative, and convenient, e.g. telemedicine methods of detection of SARS-CoV-2 that can identify a patient's past and present infection status simultaneously. DETAILED DESCRIPTION - Biosensor comprises a microfluidics layer, a multimodal layer comprising a counter electrode, a reference electrode, a first working electrode comprising a first detection protein configured to capture a first severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) biomarker. Second working electrode comprises a second detection protein configured to capture a second SARS-CoV-2 biomarker. Logic circuit comprises a processor and a non-transitory memory with computer executable instructions embedded on it. The microfluidics layer comprises multiple microchannels transversely oriented to channel a biological sample from a first surface of the microfluidics layer to a second surface of the microfluidics layer. The biological sample comprises a first and second SARS-CoV-2 biomarker. The multimodal sensing layer is fluidically coupled to the second surface of the microfluidics layer to receive the biological sample from the microchannels. The electrodes configured to detect a measurement of an electrical property corresponding to the first and second SARS-CoV-2 biomarkers being present in the biological sample. The logic circuit is electrically coupled to the electrodes and the computer executable instructions cause the processor to identify the electrical properties detected with the electrodes when the first and second SARS-CoV-2 biomarkers are present in the biological sample. An INDEPENDENT CLAIM is also included for a method for detecting SARS-CoV-2 biological markers, which involves: (1) comprising a biosensor comprising a microfluidics layer comprising a multiple of microchannels transversely oriented to channel a biological sample from a first surface of the microfluidics layer to a second surface of the microfluidics layer, a multimodal sensing layer fluidically coupled to the microfluidics layer comprising a counter electrode, a reference electrode, a first working electrode comprising a first detection protein configured to capture a first SARS-CoV-2 biomarker, and a second working electrode comprising a second detection protein configured to capture a second SARS-CoV-2 biomarker, and a logic circuit; (b) receiving, on a first surface of the microfluidics layer, a biological sample comprising the first and second SARS-CoV-2 biomarkers, such that the biological sample can be channeled from the first surface of the microfluidics layer to the second surface of the microfluidics layer; (c) obtaining, with the electrodes, a measurement of an electrical property of the first and second SARS-CoV-2 biomarkers; (d) generating, with the logic circuit, an indication that the first and second SARS-CoV-2 biomarkers are present in the biological sample based on the measurement of the electrical properties of the first and second SARS-CoV-2 biomarkers.