▎ 摘　　要

NOVELTY - Preparation of enzyme-free glucose electrochemical detection test paper involves spin-coating photoresist on the electrode substrate, patterning the photoresist to form patterned electrode substrate and plating an electrode layer on patterned electrode substrate to obtain patterned electrode layer, plating iron-cobalt-nickel/reduced graphene oxide composite film on the patterned electrode layer by electrochemical deposition to obtain preliminary detection electrode, drip-coating Nafion(RTM: sulfonated tetrafluoroethylene based fluoropolymer-copolymer) layer solution on surface of the preliminary detection electrode, adding Nafion(RTM: sulfonated tetrafluoroethylene based fluoropolymer-copolymer) and polychlorotrifluoroethylene mixed layer solution and 2-methacryloyloxyethylphosphorylcholine layer solution sequentially and drying to obtain electrochemical detection electrode, and attaching double-sided adhesive tape and a hydrophilic film to the electrochemical detection electrode. USE - Preparation of enzyme-free glucose electrochemical detection test paper. ADVANTAGE - The enzyme-free glucose electrochemical detection test paper has strong environmental anti-interference ability and stability, high sensitivity and excellent electrocatalytic performance. DETAILED DESCRIPTION - Preparation of enzyme-free glucose electrochemical detection test paper involves spin-coating photoresist on the electrode substrate, patterning the photoresist to form a patterned electrode substrate and plating an electrode layer on patterned electrode substrate to obtain a patterned electrode layer, plating an iron-cobalt-nickel/reduced graphene oxide composite film on the patterned electrode layer by electrochemical deposition to obtain a preliminary detection electrode, where the conditions of electrochemical deposition are deposition potential of 0.2-0.8 V, deposition time of 50-1000 seconds and deposition temperature of 10-50℃, drip-coating Nafion (RTM: sulfonated tetrafluoroethylene based fluoropolymer-copolymer) layer solution on surface of the preliminary detection electrode, adding Nafion (RTM: sulfonated tetrafluoroethylene based fluoropolymer-copolymer) and polychlorotrifluoroethylene mixed layer solution and 2-methacryloyloxyethylphosphorylcholine layer solution sequentially and drying to obtain an electrochemical detection electrode, where the Nafion (RTM: sulfonated tetrafluoroethylene based fluoropolymer-copolymer) layer solution, Nafion (RTM: sulfonated tetrafluoroethylene based fluoropolymer-copolymer) and polychlorotrifluoroethylene mixed layer solution and 2-methacryloyloxyethylphosphorylcholine layer solution are all dripped in amount of 1-100 uL and the drying temperature is 40-80℃, and attaching double-sided adhesive tape and a hydrophilic film to the electrochemical detection electrode. An INDEPENDENT CLAIM is included for enzyme-free glucose electrochemical detection test paper, which includes electrode substrate, and patterned electrode layer plated on the electrode substrate, where the patterned electrode layer is coated with iron-cobalt-nickel/reduced graphene oxide composite film, the surface of the iron-cobalt-nickel/reduced graphene oxide composite film is drip-coated with Nafion (RTM: sulfonated tetrafluoroethylene based fluoropolymer-copolymer) layer/Nafion (RTM: sulfonated tetrafluoroethylene based fluoropolymer-copolymer) and polychlorotrifluoroethylene mixed layer/2-methacryloyloxyethylphosphorylcholine composite layer, a hydrophilic film is attached to the Nafion (RTM: sulfonated tetrafluoroethylene based fluoropolymer-copolymer) layer/Nafion (RTM: sulfonated tetrafluoroethylene based fluoropolymer-copolymer) and polychlorotrifluoroethylene mixed layer/2-methacryloyloxyethylphosphorylcholine composite layer, and the patterned electrode layer includes bottom layer of the working electrode and the bottom layer of the counter electrode, the area of the bottom layer of the working electrode is 1-10 mm2, and the line width of the bottom layer of the counter electrode is 1-10 mm.