▎ 摘　　要

NOVELTY - Fluorescent method for detecting S1 nuclease and its inhibitor, involves forming oligonucleotide/graphene oxide compound, adding the oligonucleotide complementary chain restricted by S1 nuclease into the oligonucleotide/graphene oxide compound, performing specificity degradation on the oligonucleotide complementary chain by S1 nuclease, and qualitative and quantitative detection on the S1 nuclease, performing the degradation on the oligonucleotide complementary chain by the S1 nuclease, and forming the double-helix structure with the oligonucleotide probe. USE - The method is useful for detecting S1 nuclease and its inhibitor. ADVANTAGE - The method is simple, rapid, and has high sensitivity and selectivity. DETAILED DESCRIPTION - Fluorescent method for detecting S1 nuclease and its inhibitor, involves (a) adding the oligonucleotide probe labeled using dye into the buffer solution, performing fluorescent detection using activation wavelength of 480 nm and recording the fluorescent transmission spectrum band of the probe, (b) adding the graphene oxide into the solution, adsorbing the free curved oligonucleotide probe using the graphene oxide as solid carrier to form oligonucleotide/graphene oxide compound, where the fluorescence of the dye is quenched by the graphene oxide at the moment, (c) adding the oligonucleotide complementary chain restricted by S1 nuclease into the oligonucleotide/graphene oxide compound to perform the reaction, (d) performing specificity degradation on the oligonucleotide complementary chain by S1 nuclease to form the small base fragment and not to form the double helix structure with the oligonucleotide probe on the surface of the graphene oxide, such that the oligonucleotide probe cannot disengage from the surface of the graphene oxide and the fluorescent signal of the dye cannot be restored, and performing the qualitative and quantitative detection on the S1 nuclease based on the restoring degree of the fluorescent signal, (e) performing the degradation on the oligonucleotide complementary chain by the S1 nuclease under the presence of adenosine triphosphoric acid, adding the oligonucleotide/graphene oxide compound to perform the reaction, and (f) under the presence of adenosine triphosphoric acid, the oligonucleotide complementary chain is not degraded by the S1 nuclease, and forming the double-helix structure with the oligonucleotide probe, where the oligonucleotide probe disengages from the surface of the graphene oxide and the fluorescent signal of the dye is restored, and performing the qualitative and quantitative detection on the adenosine triphosphoric acid based on the restoring degree of the fluorescent signal.