▎ 摘　　要

NOVELTY - An alcohol detecting method involves utilizing graphene ECL biosensor under instrument detection procedures and conditions ECL Analyzer with molybdenum wire electrode, silver/silver chloride reference electrode, reagents and materials such as terpyridine ruthenium chloride hexahydrate, alcohol dehydrogenase, beta -nicotinamide adenine dinucleotide, monosodium phosphate, disodium phosphate, ammonia, hydrazine, Poly(3,4-ethylenedioxythiophene), polystyrene sulfonic acid, and graphene oxide. Circular ITO electrode is prepared and dried. Detection process is performed by using cyclic voltammogram. USE - Method for detecting alcohol. ADVANTAGE - The method enables detecting alcohol with ethanol-saving, high sensitivity, and good selectivity in simple manner. DETAILED DESCRIPTION - An alcohol detecting method involves utilizing graphene ECL biosensor under instrument detection procedures and conditions ECL Analyzer with molybdenum wire electrode, silver/silver chloride reference electrode, reagents and materials such as terpyridine ruthenium chloride hexahydrate (Ru (bpy) 32+), alcohol dehydrogenase (ADH), beta -nicotinamide adenine dinucleotide (NAD+), monosodium phosphate, disodium phosphate, ammonia, hydrazine, Poly(3,4-ethylenedioxythiophene) (PEDOT), polystyrene sulfonic acid (PSS), and graphene oxide. Related solutions is prepared by preparing NAD+ solution, a phosphate buffer solution, background solution, standard solution of ethanol, test sample solution and modified electrode solution. PEDOT-PSS mixture solution is prepared by taking 1mL of PEDOT mixed with PSS 6mL of ultrasound, formulated into PED0T-PSS mixed solution, preparing PEDOT-PSS-G (G represents graphene) mixed solution, adding PED0T-PSS mixed solution to the prepared 1.3mL 1.25mL graphene oxide, adding 40 mu l ammonia and 3.5 mu l hydrazine, mix with the secondary water in a volumetric flask dilution, the volume to 10mL, to obtain PEDOT-PSS-G mixed solution.ADH solution is prepared by dissolving 1 mg ADH in 100 mu l secondary water to obtain a solution of ADH, preparing NAD + solution, secondary water formulated with a concentration of 0.025mol / L of NAD + solution. Solution of modified electrodes is prepared by taking ADH solution in 20 mu l mixed solution of 20 mu l, preparing NAD + solution to obtain a PEDOT-PSS-G mixed together to obtain an electrode solution, preparing 32+ Ru (bpy) solution. Circular ITO electrode is prepared and dried at room temperature to obtain a composite film containing the ITO electrode, ITO electrode containing the composite membrane immersed in 0.8 mmol/L Ru (bpy) 32+ solution, soaking for 10 s after the removal, and drying at room temperature, to obtain graphene ECL biosensor, which is stored at 4 degrees C refrigerator for later use. Detection process is performed by using cyclic voltammogram of ethanol standard solution Graphene ECL biosensor experiments as the working electrode, background solution, scanning potential region is 0-1.30 V, recording stable cyclic voltammograms, using graphene ECL biosensor prepared as a working electrode, circulating ethanol standard solution for 5-10 weeks, recording cyclic voltammetry stable, circulating ethanol standard solution to obtain a solution of the background voltammogram, comparing to determine the determination of electrochemically active interval detection ethanol, detecting sample solution under electrochemiluminescence detection cell located above the photomultiplier tube, photomultiplier tube bias voltage set to 800 V, sweep potential to 0-1.25 V, adding test sample solution L ECL detection to the pool, utilizing graphene ECL biosensor as the working electrode, molybdenum wire for the pole, Ag/AgCl as reference electrode, liquor sample obtained in ethanol ECL detection pattern.