▎ 摘　　要

NOVELTY - Basal ganglia-on-a-chip comprises graphene-conjugated magnetic nanoparticles patterned on a substrate, a first hydrogel containing glutamatergic neurons and a second hydrogel containing GABAergic neurons, the first and second hydrogels being disposed in parallel on a pattern of the graphene-conjugated magnetic nanoparticles, a third hydrogel in contact with the second hydrogel, the third hydrogel containing GABAergic neurons and neuronal membrane protein-specific antibody-conjugated magnetic nanoparticles and a fourth hydrogel in contact with the third hydrogel, the fourth hydrogel containing dopaminergic neurons and neuronal membrane protein-specific antibody-conjugated magnetic nanoparticles. USE - Basal ganglia-on-a-chip used for screening therapeutic agents for brain and nervous system diseases, where the brain and nervous system diseases are dopamine-dependent brain and nervous system diseases and dopamine-dependent brain and nervous system disease are brain and nervous system disease selected from the group consisting of parkinson's disease, neurocognitive disorder, attention deficit hyperactivity disorder, restless leg syndrome, schizophrenia, and anxiety (all claimed). DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for the following: (1) a method for screening therapeutic agents for brain and nervous system diseases by using a basal ganglia-on-a-chip, which involves treating dopaminergic neurons with a candidate of therapeutic agents for brain and nervous system diseases, and investigating whether the dopaminergic neurons proliferate or are reduced; and (2) a method for fabricating a basal ganglia-on-a-chip for screening therapeutic agents for brain and nervous system diseases, which involves patterning graphene-conjugated magnetic nanoparticles on a substrate while a magnetic field is applied to the substrate in a vertical direction, printing a first bio-ink on a pattern of the graphene-conjugated magnetic nanoparticles while a magnetic field is applied to the pattern in a vertical direction, the first bio-ink containing glutamatergic neurons and a hydrogel, printing a second bio-ink on the pattern of the graphene-conjugated magnetic nanoparticles while a magnetic field is applied to the pattern in a vertical direction, such that the second bio-ink is adjacent to one side surface of a region printed with the first bio-ink, the second bio-ink containing GABAergic neurons and a hydrogel, printing a third bio-ink on a region printed with the second bio ink, the third bio-ink containing GABAergic neurons, neuronal membrane protein-specific antibody-conjugated magnetic nanoparticles, and a hydrogel, printing a fourth bio-ink on a region printed with the third bio-ink, the fourth bio-ink containing dopaminergic neurons, neuronal membrane protein-specific antibody-conjugated magnetic nanoparticles, and a hydrogel and applying a magnetic field in a vertical direction from below the substrate.