▎ 摘　　要

NOVELTY - A 3D-structured reduced graphene oxide-multi-walled carbon nanotube-palladium nanocomposite sensor preparing method involves grinding a glass carbon electrode with aluminum oxide polishing powder, adding nanocomposite solution onto an electrode surface, and drying the electrode. Porcine parvovirus antibody is added dropwise onto the electrode and dried. Bovine serum albumin solution is added dropwise onto the electrode surface and dried. Porcine parvovirus antigen standard solution is added dropwise onto the electrode surface and dried. USE - Method for preparing 3D-structured rGO-MWCNT-Pd sensor that is utilized for detecting unlabeled porcine parvovirus. ADVANTAGE - The method enables preparing 3D-structured rGO-MWCNT-Pd sensor with large specific surface area, better biological compatibility and high catalytic efficiency, and detecting unlabeled porcine parvovirus with improved sensitivity and stability. DETAILED DESCRIPTION - A 3D-structured reduced graphene oxide-multi-walled carbon nanotube-palladium nanocomposite (rGO-MWCNT-Pd) sensor preparing method involves grinding glass carbon electrode with diameter of 4 mm with aluminum oxide polishing powder, cleaning the glass carbon electrode with clean super-pure water, adding 6 mu l rGO-MWCNT-Pd solution with concentration of 0.5-2 mg/ml onto the surface of the glass carbon electrode, and drying the glass carbon electrode under room temperature. 6 mu l porcine parvovirus antibody with concentration of 5-10 mu g/ml is added dropwise onto the surface of the glass carbon electrode and dried at 4 degrees C in refrigerator. The glass carbon electrode is washed with super-pure water and dried at 4 degrees C in refrigerator. 3 mu l bovine serum albumin solution with concentration of 1-2 mass% is added dropwise onto the surface of the glass carbon electrode to close non-specific active sites on the electrode surface and dried at 4 degrees C in refrigerator. The glass carbon electrode is washed with super-pure water and dried at 4 degrees C in refrigerator. 6 mu l series of different concentrations of 0.01-25 ng/ml of porcine parvovirus antigen standard solution is added dropwise onto the surface of the glass carbon electrode and dried at 4 degrees C in refrigerator. The glass electrode is washed with super-pure water and dried at 4 degrees C in refrigerator to obtain the 3D-structured rGO-MWCNT-Pd sensor.