• 专利标题:   Method for producing chitosan-ionic liquid-graphene-enzyme composite-modified electrode, involves dissolving chitosan in diluted acid, adding graphene oxide, ionic liquid and enzyme to solution, and depositing mixture on working electrode.
  • 专利号:   CN104034778-A
  • 发明人:   LI L, WU Y, YU X, MU H, SUN P, TUO X
  • 专利权人:   WUHAN INST TECHNOLOGY
  • 国际专利分类:   G01N027/333
  • 专利详细信息:   CN104034778-A 10 Sep 2014 G01N-027/333 201480 Pages: 7 Chinese
  • 申请详细信息:   CN104034778-A CN10272483 18 Jun 2014
  • 优先权号:   CN10272483

▎ 摘  要

NOVELTY - A chitosan-ionic liquid-graphene-enzyme composite-modified electrode producing method involves dissolving chitosan in diluted acid to obtain solution A, adding graphene oxide to the solution A, dispersing mixture to obtain solution B, adding ionic liquid to the solution B, stirring mixture to obtain solution C, adding enzyme to the solution C, and treating mixture to obtain solution D. The obtained solution D is deposited on the working electrode. USE - Method for producing a chitosan-ionic liquid-graphene-enzyme composite-modified electrode. ADVANTAGE - The method enables producing the chitosan-ionic liquid-graphene-enzyme composite-modified electrode with better electron transmission channel between redox center and surface of enzyme and maintaining biological activity of enzyme. DETAILED DESCRIPTION - A chitosan-ionic liquid-graphene-enzyme composite-modified electrode producing method involves dissolving chitosan in 0.01-1 mol/l diluted acid to obtain solution A containing 0.5-5 mass%, adding 5-20 mg graphene oxide to the solution A, dispersing the mixture ultrasonically for 1-2 hours to obtain solution B, adding ionic liquid to the solution B, stirring the mixture for 1-2 hours to obtain solution C containing 1-5 mass% ionic liquid, adding enzyme to the solution C, and treating the mixture ultrasonically for 10-30 minutes to obtain solution D containing 0.01-1 mg/ml enzyme. The obtained solution D is deposited on the working electrode at constant potential to obtain a chitosan-ionic liquid-graphene-enzyme composite-modified electrode.