• 专利标题:   Preparation of biosensor used for detection of carcino-embryonic antigens, by adding silver-amino graphene-molybdenum disulfide aqueous solution to carbon electrode, drying, adding carcinoembryonic antibody, and storing electrode.
  • 专利号:   CN105353121-A, CN105353121-B
  • 发明人:   DU B, FAN D, HU L, MA H, PANG X, SUN X, WU D, YAN T, ZHANG Y, WEI Q
  • 专利权人:   UNIV JINAN, UNIV JINAN
  • 国际专利分类:   G01N033/561, G01N033/574
  • 专利详细信息:   CN105353121-A 24 Feb 2016 G01N-033/561 201646 Pages: 6 English
  • 申请详细信息:   CN105353121-A CN10987740 27 Dec 2015
  • 优先权号:   CN10987740

▎ 摘  要

NOVELTY - Method for preparation of biosensor established on basis of silver-amino graphene-molybdenum disulfide, involves polishing alumina powder on glassy carbon electrode, ultrasonic washing the polished electrode using ultrapure water and ethanol, drying, dropwisely adding silver-amino graphene-molybdenum disulfide aqueous solution to the dried electrode, drying, adding carcinoembryonic antibody (CEA), refrigerating, further adding solution of bovine serum albumin to the electrode, refrigerating, adding CEA antibodies, incubating, washing, and storing electrode. USE - The method is useful for preparation of biosensor (claimed) which is used for detection of carcino-embryonic antigens. ADVANTAGE - The method improves sensitivity of the biosensor. DETAILED DESCRIPTION - Method for preparation of biosensor established on basis of silver-amino graphene-molybdenum disulfide, involves (a) polishing 1, 0.3 or 0.05 mu m alumina powder on glassy carbon electrode, ultrasonic washing the polished electrode using ultrapure water and ethanol, drying the electrode by nitrogen, dropwisely adding 2 mg/ml silver-amino graphene-molybdenum disulfide aqueous solution to the dried electrode, drying the electrode, adding 5-20 mu g/ml carcinoembryonic antibody (CEA) to the surface of the modified electrode, refrigerating the electrode at 4 degrees C for 1 hour, further adding 3 mu l 5-20 mg/ml solution of bovine serum albumin to the electrode, refrigerating the electrode at 4 degrees C for 1 hour, adding 6 mu l 0.0002-20 ng/ml CEA antibodies to the electrode, incubating the electrode at room temperature for 1 hour, washing the electrode, and storing the electrode at 4 degrees C.