• 专利标题:   Preparation of graphene medicine nanocarrier material used in cell gene infection, by dispersing graphene oxide in ethanol, and reacting with N,N-dicyclohexylcarbodiimide and polyethyleneimine-N,O-carboxymethyl chitosan buffer solution.
  • 专利号:   CN108704140-A
  • 发明人:   ZHAO L, TANG N, LENG J, SONG C, ZHONG Y, YU P, WANG X
  • 专利权人:   UNIV GUANGXI CHINESE MEDICINE
  • 国际专利分类:   A61K047/04, A61K047/34, A61K047/36, A61K048/00, A61P035/00
  • 专利详细信息:   CN108704140-A 26 Oct 2018 A61K-047/04 201902 Pages: 9 Chinese
  • 申请详细信息:   CN108704140-A CN10595694 11 Jun 2018
  • 优先权号:   CN10595694

▎ 摘  要

NOVELTY - Preparation of a graphene medicine nanocarrier material comprises preparing graphene oxide using Hummers method; preparing branched polyethyleneimine-N,O-carboxymethyl chitosan buffer solution by dissolving hyperbranched polyethyleneimine in ethanol solution, slowly dripping 0.2% N,O-carboxymethyl chitosan, heating for volatilizing organic solvent, stirring to completely evaporate and obtain a homogeneous solution, adding the homogeneous solution to n-hexane to obtain a pale yellow precipitate, centrifuging with distilled water, and storing in a phosphate-buffered saline; and dispersing dried graphene oxide in ethanol, adding N,N -dicyclohexylcarbodiimide as condensing agent under ultrasonication, dropwise adding branched polyethyleneimine-N,O-carboxymethyl chitosan buffer solution, reacting at 45 degrees C for 24 hours, washing with ethanol, and freeze-drying. USE - Method for preparing a graphene medicine nanocarrier material used in cell gene infection (claimed). ADVANTAGE - The graphene medicine nanocarrier material has good biocompatibility and biodegradability, and low cytotoxicity. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is included for graphene medicine nanocarrier material.