▎ 摘　　要

NOVELTY - Preparation of graphene-based quantum dots electrochemical lighting sensor involves dispersing amino graphene in 50 mL ultrapurified water, ultrasonic treating for 10 hours, adding solid silver nitrate, 5 mL chloroauric solution and 5 mg polyvinyl pyrrolidone, stirring for 6 hours, adding 4 mL sodium citrate solution, stirring, centrifuging for 15 minutes, washing with ultrapurified water, centrifuging, vacuum drying at 35 degrees C, mixing amino graphene quantum dots and graphene carboxylated quantum dots, adding 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide and N-hydroxy-succinimide, and mixing. USE - Method for preparing graphene-based quantum dots electrochemical lighting sensor used for measuring prostate specific antigen (all claimed) and detecting tumor. ADVANTAGE - The sensor is stable and has high selectivity. The detection method is repeatable. DETAILED DESCRIPTION - Preparation of graphene-based quantum dots electrochemical lighting sensor comprises dispersing amino graphene in 50 mL ultrapurified water, ultrasonic treating for 10 hours, adding solid silver nitrate, 5 mL chloroauric solution and 5 mg polyvinyl pyrrolidone, stirring for 6 hours, adding 4 mL sodium citrate solution, stirring, centrifuging for 15 minutes, washing with ultrapurified water, centrifuging, vacuum drying at 35 degrees C, mixing amino graphene quantum dots and graphene carboxylated quantum dots, adding 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide and N-hydroxy-succinimide, mixing at 4 degrees C for 5 hours, shocking for 24 hours, centrifuging, washing with phosphate buffered saline solution, centrifuging, dispersing, drying, adding prostate specific antigen to electrode surface, adding bovine serum albumin solution, washing with ultrapurified water, drying, and storing at 4 degrees C. An INDEPENDENT CLAIM is included for measuring prostate specific antigen comprising taking sensor, connecting to gold/gold chloride electrode, platinum electrode and electrochemical luminescence detector, adjusting voltage to 600 V, scanning, adding potassium persulfate and phosphate buffered saline solution, determining signal intensity, drawing working curve and comparing test results.