▎ 摘　　要

NOVELTY - The method involves transferring layer of graphene to support layer to form support layer with graphene layer on top. The amphiphilic coating applied to the graphene layer to form a non-covalent surface coating on graphene layer. The device having support layer and biofunctionalized graphene layer obtained on top of the support layer. USE - Method for manufacturing graphene induced energy transfer (GIET) deviced for determining structure and/or dynamics of target analyte (all claimed). ADVANTAGE - The method provides biofunctionalization of graphene layers for site-specific immobilization of biomolecules for determination of structure or conformational changes. Using the non-covalent biofunctionalized graphene in GIET based methods, the structure of the biomolecule remains intact and their orientation can be controlled. Thus, the distance dependent GIET efficiency can be calibrated and used for high precision quantitative analysis of size and dynamics of structurally defined biomolecular, e.g. nucleotides, protein, protein complex, protein with an intrinsically disordered domain, or protein complexes with dimensions of up to 50 nm. DESCRIPTION OF DRAWING(S) - The drawing shows a graphical diagram of for manufacturing graphene induced energy transfer device. Black curve (1) Magnetically stirred (4)