▎ 摘　　要

NOVELTY - Preparation of metal-organic framework-nitrogen-doped graphene metalloenzyme involves: adding 9.9 mmol iron(III) chloride hexahydrate and 9.9 mmol terephthalic acid in molar ratio of 1:1 to 100 mL dimethylformamide (DMF), stirring to dissolve, heating and refluxing in an oil bath at 100℃ and naturally cooling; rapidly centrifuging and separating using a high-speed centrifuge, washing with DMF and ethanol and drying in vacuum oven at 70℃ for 12 hours to obtain an iron-based metal-organic framework material; grinding into fine powder and calcining under nitrogen at 600℃ for 3 hours; ultrasonically dispersing 2.5 mg nitrogen-doped graphene in 5 mL deionized water to obtain 0.5 mg/L suspension; mixing suspension with anhydrous ethanol in a volume ratio of 1:1, adding the calcined powder and dispersing, where the mass ratio of nitrogen-doped graphene to powder is 1:2; and centrifuging at 9000 rpm for 5 minutes, washing 3 times with anhydrous ethanol and drying at 70℃ for 12 hours. USE - Preparation of metal-organic framework-nitrogen-doped graphene metalloenzyme used in preparation of hydrogen peroxide detection product (all claimed). Hydrogen peroxide is used as oxidizing agent in urban sewage treatment, sterile packaging, medical and cosmetic industry. ADVANTAGE - The method is simple and low in energy consumption, does not cause harm to the environment, and provides metalloenzyme, which has catalase property, and can achieve rapid, sensitive and high selective electrochemical detection of hydrogen peroxide.