▎ 摘　　要

NOVELTY - The method involves depositing a brittle material (103) on a substrate (101), then depositing a conductive graphene layer at the surface of brittle material, and propagating a crack through the brittle material and graphene using a force. The crack forms a nano-gap. One or more indentions are placed on the brittle material to mark endpoints of the crack, prior to propagation of the crack. The substrate can be made of polyethylene terephthalate (PET), while the brittle material can be salt, ceramic, silica glass substance, and the brittle film (102) is spin on glass (SOG) cured liquid glass. USE - Development method of conductive nano-gap in strip used for DNA sequencing. ADVANTAGE - Obtains a desired width on nano-gap by being able to manipulate and stabilize substrate, thus readings made on resistive reader system can have high accuracy and resolution. Achieves large read length and high throughput simultaneously by using nano-gap-based sequencing. DESCRIPTION OF DRAWING(S) - The drawing shows a side view of strip having a substrate coated with a brittle film. Strip (100) Substrate (101) Brittle film (102) Brittle material (103)