▎ 摘　　要

NOVELTY - A reduced graphene oxide-chitosan-ferrocene/gold nanoparticles nanocomposite-based alpha -fetoprotein detecting method involves pouring graphene oxide into distilled water, dissolving and homogenizing, adding ascorbic acid and reducing graphene oxide, and adding chitosan to the acetic acid solution to obtain chitosan solution. The ferrocene is mixed with the chitosan solution, activated, and stirred, added the reduced graphene suspension, activated and centrifuged. The screen printing electrode is placed in sulfuric acid solution and performed cyclic voltammetry scanning, soaked in glutaraldehyde, incubated with reduced graphene oxide-chitosan-ferrocene suspension, washed, and air-dried, added aminated alpha fetoprotein aptamer, incubated and washed, and added the bovine serum albumin solution dropwise and dried. The working electrode is placed in phosphate-buffered saline solution, and recorded the peak current, and drawn a standard curve to obtain the concentration of alpha-fetoprotein. USE - Method for detecting reduced graphene oxide-chitosan-ferrocene/gold nanoparticles nanocomposite-based alpha -fetoprotein. ADVANTAGE - The method enables detecting the reduced graphene oxide-chitosan-ferrocene/gold nanoparticles nanocomposite-based alpha -fetoprotein with effectively realizing the quantitative detection of alpha fetoprotein, and low detection limit in simple and cost-effective manner. DETAILED DESCRIPTION - A reduced graphene oxide-chitosan-ferrocene/gold nanoparticles nanocomposite-based alpha -fetoprotein detecting method involves utilizing binding aptamer as a recognition probe, pouring graphene oxide into distilled water, ultrasonically utilizing ultrasonic cell crusher to make it fully dissolved and homogenizing to prepare graphene oxide aqueous solution, taking the above graphene oxide aqueous solution, adding ascorbic acid and reducing graphene oxide to obtain reduced graphene oxide, and adding chitosan to the acetic acid solution to obtain chitosan solution. The ferrocene is mixed with the above chitosan solution, activated by carbodiimide/N-hydroxysuccinimide, and stirred to obtain chitosan-ferrocene complex. The reduced graphene suspension is added to the chitosan-ferrocene solution, activated carbodiimide/N-hydroxysuccinimide and centrifuged to obtain reduced graphene oxide-ferrocene suspension. The screen printing electrode is placed in sulfuric acid solution and performed cyclic voltammetry scanning to obtain activated screen-printed electrode. The activated screen printing electrode is placed in a chloroauric acid solution for constant potential deposition, after completing the deposition, rinsed the electrode with water to obtain a gold nanoparticles/screen printed electrode electrode. The gold nanoparticles/screen printed electrode is soaked in glutaraldehyde, washed with phosphate-buffered saline, and blown dried, incubated with reduced graphene oxide-chitosan-ferrocene suspension, washed with phosphate-buffered saline, and air-dried to obtain reduced graphene oxide-chitosan-ferrocene/gold nanoparticles/screen printed electrode. The aminated alpha fetoprotein aptamer is added to the sensor interface, incubated for a period of time, and washed the alpha-fetoprotein aptamer not fixed to the interface with phosphate-buffered saline solution, and added the bovine serum albumin solution dropwise to obtain alpha fetoprotein aptamer/reduced graphene oxide-chitosan-ferrocene/gold nanoparticles/screen printed electrode sensing interface, dried for use, which is added with standard alpha fetaprotein solution dropwise, incubated, washed with phosphate-buffered saline solution to obtain a working electrode, and dried for further use. The working electrode is placed in phosphate-buffered saline solution, and recorded the peak current by a DPV scan of an electrochemical workstation, tested different concentrations of alpha-fetoprotein, and drawn a standard curve to calculate the minimum detection limit of the method, added the actual sample to be tested, incubated, washed with phosphate-buffered saline solution to obtain working electrode and dried for use. The working electrode is placed in phosphate-buffered saline solution, and recorded the peak current by a differential pulse voltammetry scan of an electrochemical workstation to obtain the concentration of alpha-fetoprotein in the actual sample to be tested according to the standard curve.