▎ 摘　　要

NOVELTY - Reagent combination comprises first conjugate, second conjugate, substrate protein binder, second nucleic acid molecule, third conjugate, and oxidation inhibitory reagent, where first conjugate is formed by coupling first nucleic acid molecule and antibody, where first nucleic acid molecule contains first hybridization region and second hybridization region and antibody specifically binds to a small molecule substance. The second conjugate is formed by sequentially coupling a substrate protein binder, and second nucleic acid molecule and a second fluorescent group. The substrate protein binder is formed by coupling a small molecular substrate and a scaffold protein, so that the small molecule substrate is the same as the small molecule substance. USE - Reagent combination used in small molecule substance detection kit and system for detecting small molecular substances. ADVANTAGE - The reagent combination ensure that the method of detection of small molecules is simple, background interference is small, sensitivity is high, measuring error is small. DETAILED DESCRIPTION - Reagent combination comprises first conjugate, second conjugate, substrate protein binder, second nucleic acid molecule, third conjugate, and oxidation inhibitory reagent, where first conjugate is formed by coupling first nucleic acid molecule and antibody, where first nucleic acid molecule contains first hybridization region and second hybridization region and antibody specifically binds to a small molecule substance. The second conjugate is formed by sequentially coupling a substrate protein binder, and second nucleic acid molecule and a second fluorescent group. The substrate protein binder is formed by coupling a small molecular substrate and a scaffold protein, so that the small molecule substrate is the same as the small molecule substance. The second nucleic acid molecule has a third hybridization region and fourth hybridization region, where the third hybridization region is complementary to the second hybridization region. The third conjugate is formed by coupling a first fluorescent group and a third nucleic acid molecule. The third nucleic acid molecule comprises fifth hybridization region and sixth hybridization region, and fifth hybridization region and fourth The hybridization region is complementary, and sixth hybridization region is complementary to the first hybridization region. The first fluorescent group binds to the small molecule substrate in the antibody and the substrate protein conjugate and under the condition of being oxidized by an oxidant, a first fluorescence is emitted, and the first fluorescence excites the second fluorescent group under the condition that the first nucleic acid molecule. The second nucleic acid molecule and third nucleic acid molecule are paired with each other emit a second fluorescence to obtain the content of the small molecular substance according to the intensity of the second fluorescence and one-to-one correspondence between the fluorescence intensity and the content of the small molecular substance. The oxidation inhibitory reagent includes an antioxidant for inhibiting the oxidation of the first fluorescent group to emit the first fluorescence. INDEPENDENT CLAIMS are included for: Method for detecting small molecular substances, which involves adding first conjugate, second conjugate, third conjugate and oxidation inhibitory reagent to the solution to be tested, and mixing to form a sample to be tested, where first conjugate comprises a first nucleic acid molecule and an antibody coupled to The antibody can specifically bind to small molecular substances; second conjugate is formed by sequentially coupling a substrate protein binder, a second nucleic acid molecule and a second fluorescent group, and the substrate The protein conjugate is formed by coupling a small molecular substrate and a scaffold protein, and the small molecular substrate is the same as the small molecular substance; third conjugate is coupled by a first fluorescent group and a third nucleic acid molecule under the condition that the solution to be tested does not contain or contains a small amount of the small molecule substance, the antibody and the substrate protein conjugate form an immune complex, and first nucleic acid molecule, first nucleic acid molecule, second nucleic acid molecule and third nucleic acid molecule forms a neck ring structure, and the first fluorescent group and the second fluorescent group are located on the same side of the neck ring structure; oxidation-inhibiting reagent for inhibiting the oxidation of the first fluorescent group is removed from the sample to be tested, an oxidizing agent for oxidizing the first fluorescent group to emit first fluorescence is added, and according to the first fluorescent group, and maximum emission wavelength of the two fluorophores collects the second fluorescence, when the second fluorescence is collected, content of the small molecular substance is obtained according to the intensity of the second fluorescence and the one-to-one correspondence between the fluorescence intensity and the content of the small molecular substance. Small molecule substance detection kit, which comprises first storage tube stores, which the conjugate of the first nucleic acid molecule and the antibody, and the antibody specifically bind to the small molecule substance; where second storage tube stores the conjugate of the substrate protein conjugate, second nucleic acid molecule and second fluorescent group; substrate protein conjugate is formed by coupling a small molecular substrate and a scaffold protein, and small molecular substrate, where substance is the same as the small molecule substance; antibody and the substrate protein conjugate forms an immune complex in the absence or presence of a small amount of the small molecule substance in the solution to be tested; third storage tube stores the conjugate of the first fluorescent group and the third nucleic acid molecule; and Small molecule substance detection system, which comprises reaction vessel, which has a accommodating chamber capable of accommodating the solution to be tested; a micro-injection pump, communicated with the accommodating chamber through an injection pipeline, and injects the mixture of the first conjugate, the second conjugate, the third conjugate and the oxidation inhibitory agent into the said accommodating chamber through the injection pipeline, where first conjugate is formed by coupling a first nucleic acid molecule and an antibody, and the second conjugate is composed of a substrate protein conjugate, a second nucleic acid molecule and a second fluorescent group in sequence. DESCRIPTION OF DRAWING(S) - The drawing shows schematic view of the structure of immune complex and neck ring of reagent combination. 1Small molecular substance 2Antibody 3Substrate protein conjugate 4First nucleic acid molecule 5Second nucleic acid molecule 6Third nucleic acid molecule 7First fluorescent group 8Second fluorescent group