▎ 摘　　要

NOVELTY - Preparing enzyme-immobilized monolithic microreactor involves utilizing nanomaterial made nano particles of gold, silver and silicon dioxide particles, carbon nanotubes and graphene, magnetic iron oxide, metal-organic framework materials or covalent organic frameworks, where the size of utilized components range from 5-1000 nanometer. The synthetic organic polymer for monolithic column, is made by reacting 5-30 wt.% functional monomeric unit with 5-30 wt.% crosslinking agent and 30-80 wt.% porogen in a stainless steel column. USE - Method for preparing enzyme-immobilized monolithic microreactor by nanomaterial-biological interaction (claimed). ADVANTAGE - The method enables to prepare enzyme-immobilized monolithic microreactor in a simple and highly efficient manner. The immobilized enzyme exhibits high catalytic activity. DETAILED DESCRIPTION - Preparing enzyme-immobilized monolithic microreactor involves utilizing nanomaterial made nano particles of gold, silver and silicon dioxide particles, carbon nanotubes and graphene, magnetic iron oxide, metal-organic framework materials or covalent organic frameworks, where the size of utilized components range from 5-1000 nanometer. The synthetic organic polymer for monolithic column, is made by reacting 5-30 wt.% functional monomeric unit with 5-30 wt.% crosslinking agent and 30-80 wt.% porogen in a stainless steel column. 0.2-5 wt.% Photoinitiator is added to the stainless steel column and then performing reaction at 45-85 degrees C for 6-48 hours. The tip portion and the syringe body that performs capillary action, are made of stainless steel column, and the main body portion is made of borosilicate glass and porous silicon wafers to form synthetic organic polymer for monolihic column. The functional monomeric unit is selected from glycidyl methacrylate, methyl methacrylate, butyl methacrylate, lauryl methacrylate, acrylic acid, acrylamide, N-isopropylacrylamide, glycidyl propargyl ether, 4,4-dimethyl-2-vinyl-2-oxazoline-5-one, 2-methyl-2-propenoic acid, 2-hydroxypropyl methacrylate, styrene or 4-vinylpyridine. Crosslinking agent is selected from ethylene glycol dimethylacrylate, triallyl cyanurate, divinylbenzene or N,N'-methylenebisacrylamide. Porogen is selected from toluene, n-heptane, cyclohexanol, dodecanol, n-propanol, 1,4-butylene glycol or polyethylene glycol of multiple molecular weights. Photonitiator is selected from azobisisobutyronitrile, 2,2-dimethoxy-2-phenylacetophenone or 2-hydroxy-2-methyl-1-phenyl acetone. The formed synthetic organic polymer for monolihic column is reacted with enzyme to obtain enzyme-immobilized monolithic microreactor as product.