▎ 摘 要
NOVELTY - Electrochemical biosensor working electrodes are loaded with a covering layer formed by antibody dispersion. The antibody dispersion includes magnetic bead-labeled monoclonal antibody corresponding to the protein to be detected and the dispersion liquid, and the dispersion liquid includes acetic acid solution, chitosan, nitrogen-doped graphene, and water. USE - Electrochemical biosensor working electrodes for use in electrochemical biosensor for detecting proteins (claimed). ADVANTAGE - The working electrodes have higher sensitivity, better stability, more uniform antibody reaction, and is more resistant to cleaning. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for: (1) a method for preparing the electrochemical biosensor working electrodes, which involves: (a) grinding, polishing and drying the surface of the screen-printed electrode to obtain a pretreated electrode; (b) uniformly covering the surface of the glassy carbon electrode with the antibody dispersion solution, drying at room temperature, washing with double distilled water, and drying to obtain the working electrode; and (2) an electrochemical biosensor, which comprises the working electrode.