• 专利标题:   Electrochemical biosensor working electrodes for use in electrochemical biosensor for detecting proteins, are loaded with covering layer formed by antibody dispersion, where antibody dispersion including magnetic bead-labeled monoclonal antibody and dispersion liquid including acetic acid solution.
  • 专利号:   CN115932005-A
  • 发明人:   ZHAO Y, XIE B, HU C, ZHENG S
  • 专利权人:   ZHEJIANG FENGNENG PHARM TECHNOLOGY CO
  • 国际专利分类:   G01N027/30, G01N027/327
  • 专利详细信息:   CN115932005-A 07 Apr 2023 G01N-027/327 202344 Chinese
  • 申请详细信息:   CN115932005-A CN11568626 08 Dec 2022
  • 优先权号:   CN11128935

▎ 摘  要

NOVELTY - Electrochemical biosensor working electrodes are loaded with a covering layer formed by antibody dispersion. The antibody dispersion includes magnetic bead-labeled monoclonal antibody corresponding to the protein to be detected and the dispersion liquid, and the dispersion liquid includes acetic acid solution, chitosan, nitrogen-doped graphene, and water. USE - Electrochemical biosensor working electrodes for use in electrochemical biosensor for detecting proteins (claimed). ADVANTAGE - The working electrodes have higher sensitivity, better stability, more uniform antibody reaction, and is more resistant to cleaning. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for: (1) a method for preparing the electrochemical biosensor working electrodes, which involves: (a) grinding, polishing and drying the surface of the screen-printed electrode to obtain a pretreated electrode; (b) uniformly covering the surface of the glassy carbon electrode with the antibody dispersion solution, drying at room temperature, washing with double distilled water, and drying to obtain the working electrode; and (2) an electrochemical biosensor, which comprises the working electrode.