▎ 摘　　要

NOVELTY - Preparation of porous microneedle patch modified by aptamer molecular probe involves formulating polydimethylsiloxane and curing agent into polydimethylsiloxane to be cured, transferring to surface of positive template of ethoxylated trimethylolpropane triacrylate microneedle array, curing, obtaining polydimethylsiloxane pinhole microneedle array template, coating the surface of pore-forming agent with a layer of bio-adhesive, preparing pore-forming material, mixing with microneedle raw material solution, pouring into polydimethylsiloxane pinhole microneedle array template, solidifying, obtaining microneedle patch containing pore-forming material, reacting with pore-forming etchant, obtaining porous microneedle patch, reacting porous microneedle patch with aptamer molecular probe in buffer solution, attaching aptamer molecular probe to pore wall of porous microneedle patch using viscosity of bioadhesive, and using sealing liquid for sealing treatment. USE - Preparation of porous microneedle patch modified by aptamer molecular probe e.g. small single-stranded DNA molecule in endotoxin detection (claimed). ADVANTAGE - The method is simple and convenient, and has high efficiency. DETAILED DESCRIPTION - Preparation of porous microneedle patch modified by aptamer molecular probe involves (1) formulating polydimethylsiloxane and curing agent into polydimethylsiloxane to be cured, transferring the polydimethylsiloxane to be cured to surface of positive template of ethoxylated trimethylolpropane triacrylate microneedle array, vacuuming, heating and curing at 70-80 degrees C, peeling off after cooling, obtaining polydimethylsiloxane pinhole microneedle array template, (2) coating the surface of pore-forming agent with a layer of bio-adhesive, preparing pore-forming material, (3) mixing microneedle raw material solution and the pore-forming material into pore-forming liquid, pouring the pore-forming liquid and the microneedle raw material solution into polydimethylsiloxane pinhole microneedle array template step by step, solidifying pore-forming liquid and the microneedle raw material solution, peeling off, obtaining microneedle patch containing pore-forming material, (4) reacting microneedle patch containing the pore-forming material with pore-forming etchant, causing holes, obtaining porous microneedle patch, (5) reacting porous microneedle patch and aptamer molecular probe in a buffer solution, attaching aptamer molecular probe to the pore wall of the porous microneedle patch using viscosity of bioadhesive, and using sealing liquid for sealing treatment. In step (4), the pore-forming agent is microsphere particles that can be corroded by the pore-forming etchant.