▎ 摘　　要

NOVELTY - Preparing electrochemical immune sensor involves performing ultrasonically dispersing graphene oxide in aqueous solution to obtain graphene oxide colloidal solution, dissolving glucose in graphene oxide colloidal solution to obtain solution A, stirring ammonia hydrate and silver nitrate solution to react until precipitate in solution disappears, mixing and stirring solution B' and solution A, standing, centrifuging and washing, collecting sediment to obtain silver-graphene oxide (Ag-GO) nano composite material, dispersing Ag-GO nano composite material in Nafion-ethanol solution to obtain Ag-GO-solution, adding Ag-GO-solution to surface of gold electrode, drying to obtain gold electrode modified by Ag-GO-solution, placing gold electrode modified by Ag-GO-in chloroauric acid (HAuCl), performing constant potential deposition to obtain gold electrode modified by silver nanoparticles (AuNPs)/Ag-GO-solution. USE - Method for preparing electrochemical immune sensor used for detecting sulfadimethoxine (claimed). ADVANTAGE - The method enables to prepare electrochemical immune sensor, which has very high selectivity for SDM and high detection accuracy. DETAILED DESCRIPTION - Preparing electrochemical immune sensor involves performing ultrasonically dispersing graphene oxide in aqueous solution to obtain graphene oxide colloidal solution, dissolving glucose in graphene oxide colloidal solution to obtain solution A, stirring ammonia hydrate and silver nitrate solution to react until precipitate in solution disappears, mixing and stirring solution B' and solution A, standing, centrifuging and washing, collecting sediment to obtain silver-graphene oxide (Ag-GO) nano composite material, dispersing Ag-GO nano composite material in Nafion-ethanol solution to obtain Ag-GO-solution, adding Ag-GO-solution to surface of gold electrode, drying to obtain gold electrode modified by Ag-GO-solution, placing gold electrode modified by Ag-GO-in chloroauric acid (HAuCl), performing constant potential deposition to obtain gold electrode modified by silver nanoparticles (AuNPs)/Ag-GO-solution, adding anti-sulfadimethoxine (SDM) solution to surface of gold electrode modified by AuNPs/Ag-GO-solution, incubating at 37 degrees C to obtain gold electrode modified by anti-SDM/AuNPs/Ag-GO-solution, adding bovine serum albumin (BSA) solution to surface of gold electrode modified by anti-SDM/AuNPs/Ag-GO-solution, incubating at 37 degrees C to obtain gold electrode modified by bovine serum albumin (BSA)/anti-SDM/AuNPs/Ag-GO-solution. An INDEPENDENT CLAIM is included for a electrochemical immunoassay method for sulfadimethoxine, which involves: (A) adding sample contains sulfadimethoxine to surface of electrochemical immunosensor; and (B) performing differential pulse voltammetry detection.