• 专利标题:   New cellobiose dehydrogenase comprising nucleotide sequence with 80% identity, capable of expressing cellobiose dehydrogenase in Pichia pastors, for use in recombinant expression vector, lactose biosensor, and lactose detection or preparation of lactose biosensor.
  • 专利号:   CN115747182-A
  • 发明人:   LIU Q, MENG Q, YANG Y, WANG B, ZHU S, ZHANG Z, MA Y, GONG W
  • 专利权人:   SHANDONG BIOLOGY INST
  • 国际专利分类:   C12N001/19, C12N015/81, C12N009/04, C12Q001/00, C12Q001/32, C12R001/84
  • 专利详细信息:   CN115747182-A 07 Mar 2023 C12N-009/04 202326 Chinese
  • 申请详细信息:   CN115747182-A CN11482286 24 Nov 2022
  • 优先权号:   CN11482286

▎ 摘  要

NOVELTY - Cellobiose dehydrogenase, is new, where the nucleotide sequence encoding the cellobiose dehydrogenase is selected from nucleotide sequence of (SEQ ID NO: 2) with 80 % identity, where sequence is not defined, which is capable of expressing the cellobiose dehydrogenase in the Pichia pastoris. USE - Cellobiose dehydrogenase for use in lactose detection or preparation of lactose biosensor (claimed). ADVANTAGE - The cellobiose dehydrogenase effectively promotes rapid development of the lactose biosensor, and has good actual application value. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for: (1) a recombinant expression vector, which comprises the nucleotide for encoding the cellobiose dehydrogenase; (2) a host cell, which comprises the recombinant vector or the host cell capable of expressing the cellobiose dehydrogenase; (3) a lactose biosensor, which comprises the cellobiose dehydrogenase; (4) a method for electrochemically measuring concentration or presence of lactose, which involves: (a) introducing the lactose biosensor and the liquid sample with or suspected of having contact; and (b) measuring the response current intensity of lactose and analyzing the concentration or presence of lactose.