▎ 摘　　要

NOVELTY - Preparing bovine serum albumin-graphene oxide modified chitosan nano drug-loading system comprises (1) preparing oxidized graphene modified chitosan by weighing graphene oxide and chitosan, adding into a solvent I, heating, carrying out ultrasonic treatment, adding condensing agent under stirring conditions, stirring, carrying out reduced pressure distillation, adding a solvent II, stirring, filtering, washing the filter cake with ethanol, and carrying out vacuum drying to obtain graphene oxide modified chitosan, (2) preparing bovine serum albumin-graphene oxide modified chitosan nanocarrier by adding acetic acid, heating, stirring, cooling, adding glutaraldehyde aqueous solution into the reaction solution, stirring, ultrasonically dispersing, filtering, washing the obtained solid with detergent, and drying to obtain bovine serum albumin-graphene oxide modified chitosan nano-carrier. USE - The method is useful for preparing bovine serum albumin-graphene oxide modified chitosan nano drug-loading system. ADVANTAGE - The method not only improves the pharmacokinetics and pharmacodynamics properties of the material, optimizes the delivery of the medicine in the viable cell, has obvious protection effect to graphene oxide modified chitosan composite material, improves the stability of the medicine in the body, The system can be added with two kinds of anti-tumor medicine to the nano carrier, paclitaxel and camptothecin, can play a synergistic effect, fully exert the medicinal activity of the two drugs. DETAILED DESCRIPTION - Preparing bovine serum albumin-graphene oxide modified chitosan nano drug-loading system comprises (1) preparing oxidized graphene modified chitosan by weighing graphene oxide and chitosan, adding into a solvent I, heating to 25-45 degrees C, carrying out ultrasonic treatment for 5-15 minutes, adding a condensing agent under stirring conditions, stirring at 25-45 degrees C for 24-72 hours, carrying out reduced pressure distillation till the volume of the reaction liquid is 1/4-1/2, adding a solvent II, stirring for 20-40 minutes, filtering, washing the filter cake with ethanol, and carrying out vacuum drying to obtain graphene oxide modified chitosan, (2) preparing bovine serum albumin-graphene oxide modified chitosan nanocarrier by adding acetic acid, heating to 50-70 degrees C, stirring for 10-30 minutes, cooling to 20-30 degrees C, adding glutaraldehyde aqueous solution into the reaction solution, stirring for 2-4 hours, ultrasonically dispersing for 20-40 minutes, filtering, washing the obtained solid with detergent, and drying to obtain bovine serum albumin-graphene oxide modified chitosan nano-carrier, (3) preparing bovine serum albumin-graphene oxide modified chitosan nano drug-loading system by weighing paclitaxel and camptothecin, adding to dimethyl sulfoxide, stirring and dissolving, the control temperature is between 20 degrees C and 30 degrees C, adding the bovine serum albumin-graphene oxide modified chitosan nanocarrier obtained in the step (2), dropwise adding water under the stirring condition, dropwise adding water for 1-3 drops/second, stirring for 0.5-1.5 hours after dropwise adding, performing ultrasonic treatment for 10-30 minutes to obtain a solution, dialyzing the obtained solution with a dialysis bag having a molecular weight of 3500 for 24-48 hours, and performing freeze drying at-50-70 degrees C to obtain a bovine serum albumin-graphene oxide modified chitosan nano drug-loading system; the solvent is dimethyl sulfoxide or N, N-dimethylformamide, the condensing agent is 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride or N, N-diisopropylcarbodiimide, the solvent II is ethanol or methanol, the solvent III is water, the glutaraldehyde aqueous solution has a mass concentration of 20-40%; the detergent is an ethanol water mixed solution, where the volume ratio of ethanol to water is 1-2: 1, the ratio of graphene oxide, chitosan, solvent I, condensing agent, solvent II, ethanol, bovine serum albumin, solvent III, acetic acid, glutaraldehyde solution, detergent, paclitaxel, camptothecin, dimethyl sulfoxide and water is 0.2-0.3: 1-2:200-300: 0.1-0.2: 200-300: 10-20: 0.3-0.8: 270-380: 0.1-0.4: 0.01-0.05: 5-10: 0.1-0.15:0. 1-0.15:50-100: 300-400.