▎ 摘　　要

NOVELTY - Performing single-molecule fluorescence detection technology based on nano-up-conversion materials involves using detection kit and an object to be detected. The detection kit includes a hollow grid-shaped detection kit formed by a substrate carrier, a fluorescent marker probe, and a hollow grid mask. The fluorescent marker probes are nano-scale up-conversion materials with fluorescent properties. A marker probe with a specific active molecule modified on the surface of the particle, and its active molecule is an antigen or antibody that can specifically bind to the detected object. The fluorescent marker probes are uniformly formed on the surface of the substrate carrier through a surface treatment process. The hollow grid mask are covered on the surface of the substrate carrier to form a hollow grid to install the detection kit, and record the number of hollow grids as W. USE - Method for performing single-molecule fluorescence detection technology based on nano-up-conversion materials. ADVANTAGE - The method enables to perform single-molecule fluorescence detection technology based on nano-up-conversion materials, which realizes the automatic detection of absolute qualitative inspection results. DETAILED DESCRIPTION - Performing single-molecule fluorescence detection technology based on nano-up-conversion materials involves using detection kit and an object to be detected. The detection kit includes a hollow grid-shaped detection kit formed by a substrate carrier, a fluorescent marker probe, and a hollow grid mask. The fluorescent marker probes are nano-scale up-conversion materials with fluorescent properties. A marker probe with a specific active molecule modified on the surface of the particle, and its active molecule is an antigen or antibody that can specifically bind to the detected object. The fluorescent marker probes are uniformly formed on the surface of the substrate carrier through a surface treatment process. The hollow grid mask are covered on the surface of the substrate carrier to form a hollow grid to install the detection kit, and record the number of hollow grids as W. The detected substance is dropped on the surface of the detection kit, it combines with the fluorescent marker probe and adsorbs on the hollow grid to form a specific detection. The detection kit is put under a fluorescence microscope, and CCD is used to automatically detect and scan to compare the difference in the luminescent refractive index of the empty grid fluorescent markers and count them for comparison. The amount of fluorescent label that is not bound to the detection substance is recorded as W1. The number of fluorescent labels bound to the test substance is recorded as W2, so as to realize the automatic detection of absolute qualitative test results.