▎ 摘　　要

NOVELTY - Extracting muscle fatigue detection and electric regulation by electrochemical interstitial fluid involves using the prepared metal mesh electric stimulation patch based on reverse ion introduction technology, applying bipolar pulse to the skin, realizing non-invasive extraction of the skin interstitial fluid, building a lactic acid modified based on nitrogen-doped graphene, hydrogen ion, ammonium ion multi-channel sensing electrode, using multi-channel sensing electrode, electrochemical detection combined with timing current and open circuit voltage, detecting the extracted skin liquid analyzing and judging the detection result, using electrical stimulation patch for bipolar pulse local electrical stimulation of muscle the electric control effect of muscle fatigue. USE - Method for extracting muscle fatigue detection and electric regulation by electrochemical interstitial fluid. ADVANTAGE - The method provides rapid and accurate detection of muscle fatigue in the current stage, muscle fatigue marker lactic acid, hydrogen ion, detection result of ammonium ion after intelligent analysis, comprehensively judging the muscle fatigue degree, and the muscle for different degrees of electrical stimulation control. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is included for an electrochemical intermedium liquid extracting muscle fatigue detecting device which comprises the microcontroller acts on the scheduling, interrupt execution, data processing, the signal generating module is used for generating the voltage excitation with different waveforms by the digital-to- analogue conversion chip, the signal collecting module is used for collecting the signal by the analogue-to- digital conversion chip and sending to the microcontroller for processing and sending, mesenchymal liquid extracting unit based on reverse osmosis extraction technology using constant current module to generate a constant micro current applied to the electric stimulation patch to realize the extraction of the skin liquid, the open-circuit voltage detection unit detects the weak voltage based on the differential amplifier, bipolar open-circuit voltage detection capability with double-channel, realizing detection of weak open-circuit voltage generated by the ion selective membrane, the timing current detecting unit is based on the constant potential rectifier circuit, controlling the potential difference between the working electrode and the reference electrode at the same time, collecting the current signal of the working electrode and the counter electrode path, the current signal is converted by current-voltage through the transimpedance amplifier, an electrical stimulation module, the digital-to- analogue conversion module controls the output of the bipolar voltage waveform, the resistance voltage amplification factor is adjusted, the electrical stimulation skin different degrees is given, the Bluetoothmodule receives the command of the upper computer program or the microcontroller and is responsible for the wireless transmission of the data.