▎ 摘　　要

NOVELTY - Label-free detection of bacteria involves fabricating a graphene field effect transistor (G-FET); synthesizing a peptide probe capable of recognizing and binding to a bacterial target; integrating the peptide probe on the G-FET to provide a G-FET/peptide device; performing electric-field assisted binding of at least one bacterial cell of the bacterial target to the G-FET/peptide device; and electrically detecting the binding of the bacterial cell to the G-FET/peptide device. USE - The method is useful for label-free detection of bacteria (claimed). ADVANTAGE - The method incorporates G-FETs, since they are highly sensitive to chemical/biological modification, can have fast detection times and can be placed on different substrates. By integrating specific peptide probes over G-FETs, the method for species and strain specific label-free detection of clinical strains of pathogenic bacteria, has high specificity and sensitivity. The method is scalable, cheap, and does not require expensive equipment to operate, unlike methods based on culture and optical detection. In addition, the use of electric field assisted attachment enables higher sensitivity and speed. The device also can be stored at room temperature and prepared without significant expertise. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is included for a label-free bacteria detection system, comprising G-FET/peptide device comprising a peptide probe capable of recognizing and binding to a bacterial target integrated on the G-FET.