▎ 摘　　要

NOVELTY - Detecting GPC3 based on RGO-CS-Fc/Pt-PdNPs nanocomposite material involves preparing RGO-CS-Fc material by pouring graphene oxide into distilled water, and using an ultrasonic cell disruptor to sonicate it to make it fully dissolved and uniform to make GO aqueous solution. The above-mentioned GO aqueous solution is taken and placed it in a beaker, and adding ascorbic acid to reduce GO to obtain RGO, adding chitosan to acetic acid solution to obtain chitosan solution, mixing ferrocene formic acid with chitosan solution, activateing with EDC/NHS, and stirring to obtain CS-Fc complex, taking RGO suspension and add it to CS-Fc solution, activate EDC/NHS, and centrifuging to obtain RGO-CS-Fc suspension. The screen-printed electrode in the H2SO4 solution is placed and performed cyclic voltammetric scanning to obtain the activated screen-printed electrode, which is rinsed with water. USE - Method for detecting GPC3 based on RGO-CS-Fc/Pt-PdNPs nanocomposite material. ADVANTAGE - The method enables to detect GPC3 based on RGO-CS-Fc/Pt-PdNPs nanocomposite material is simple, time-saving, cost-effective and has a low detection limit. DETAILED DESCRIPTION - Detecting GPC3 based on RGO-CS-Fc/Pt-PdNPs nanocomposite material involves preparing RGO-CS-Fc material by pouring graphene oxide into distilled water, and using an ultrasonic cell disruptor to sonicate it to make it fully dissolved and uniform to make GO aqueous solution. The above-mentioned GO aqueous solution is taken and placed it in a beaker, and adding ascorbic acid to reduce GO to obtain RGO, adding chitosan to acetic acid solution to obtain chitosan solution, mixing ferrocene formic acid with chitosan solution, activateing with EDC/NHS, and stirring to obtain CS-Fc complex, taking RGO suspension and add it to CS-Fc solution, activate EDC/NHS, and centrifuging to obtain RGO-CS-Fc suspension. The screen-printed electrode in the H2SO4 solution is placed and performed cyclic voltammetric scanning to obtain the activated screen-printed electrode, which is rinsed with water. The activated screen-printed electrode is placed in a solution of chloroplatinic acid and palladium nitrate for constant potential deposition, after the deposition is complete, rinse the electrode with water to obtain a Pt-PdNPs/SPE electrode. The Pt-PdNPs/SPE electrode is soaked with glutaraldehyde, washed with PBS, blow dry, then added dropwise RGO-CS-Fc suspension to incubate for a period of time, wash with PBS, and dried to obtain RGO-CS-Fc /Pt-PdNPs/SPE electrode. The aminated GPC3 aptamer dropwise is added to the sensor interface, incubated for a period of time, wash the GPC3 aptamer not fixed to the interface with PBS solution, and added BSA solution dropwise to block to obtain GPC3apt/RGO-CS-Fc /Pt-PdNPs/SPE sensor interface, and dried for use. The standard GPC3 solution dropwise is added to obtained GPC3apt/RGO-CS-Fc/Pt-PdNPs/SPE sensing interface, incubated for a period of time, wash with PBS solution to get the work electrode, dry for later use. The working electrode is put in the PBS solution, used the DPV scan of the electrochemical workstation to record its peak current to detect GPC3 at different concentrations, drawn a standard curve, and calculated the minimum detection limit of the method. The detection of GPC3 in obtained actual samples GPC3apt/RGO-CS-Fc/Pt-PdNPs/SPE sensing interface, dropped the actual sample to be tested, incubated for a period of time, and wash with PBS solution to get the working electrode and dried it for later use. The working electrode is put in the phosphate-buffered saline (PBS) solution, use the DPV scan of the electrochemical workstation to record its peak current to obtain the concentration of GPC3 in the actual sample to be tested according to the standard curve.