▎ 摘　　要

NOVELTY - An absolute molecular imprinted sensor producing method involves preparing nano silver/graphene oxide modified liquid by dispersing ammonia water solution, silver nitrate and graphene oxide, in a reactor uniformly in an ultrasonic condition at room temperature, adding glucose, stirring and reacting to obtain the nano silver oxide/graphene modified liquid. Nano-silver oxide/graphene modified electrode is prepared by polishing the surface of a glassy carbon electrode by polishing powder. The electrode is ultrasonically cleaned, washed with ethanol and dried. The surface of the glassy carbon electrode is coated with nano silver/oxide graphene modified liquid, dried under an infrared lamp, and volatilized the solvent to obtain nano silver/oxide graphene modified electrode. The absolute molecular imprinted polymer is dispersed in chitosan acetic acid solution and dropped in nano silver oxide/graphene modified electrode, followed by placing the infrared lamp to volatilize the solvent. USE - Method for producing absolute molecular imprinted sensor. ADVANTAGE - The method enables producing the absolute molecular imprinted sensor with high sensitivity and selectivity, better specificity, rapid detection. The sensor can be used repeatedly. DETAILED DESCRIPTION - An absolute molecular imprinted sensor producing method involves preparing nano silver/graphene oxide modified liquid by dispersing 85-95 mass% ammonia water solution with concentration of 5 mass%, 1.0-3.0 mass% silver nitrate and 2-6 mass% graphene oxide, in a reactor uniformly in an ultrasonic condition for 30 min at room temperature, adding 2-7 mass% glucose, stirring and reacting for 20 min at room temperature to obtain the nano silver oxide/graphene modified liquid. Nano-silver oxide/graphene modified electrode is prepared by polishing the surface of a glassy carbon electrode by 0.3 mu m, 0.01 mu m polishing powder. The electrode is ultrasonically cleaned using secondary distilled water, washed with ethanol and dried. The surface of the glassy carbon electrode is coated with 15-18 mu l nano silver/oxide graphene modified liquid, dried under an infrared lamp, and volatilized the solvent to obtain nano silver/oxide graphene modified electrode. Absolute molecular imprinted polymer is prepared by adding 76-82 mass% ethanol, 8-12 mass% N-allyl-2-aminomethyl-pyrrolidine, 4-8 mass% 4-amino-pyridine, 2-5 mass% prometryn, 0.5-1.5 mass% diisocyanate and 0.5-1.5 mass% azo-bis-iso-butyrynitrile in the reactor, stirring to dissolve, infusing nitrogen oxygen for 15 min in oxygen free atmosphere at 55 plus minus 2 degrees C to obtain product. The product is stirred for 10-12h and reacted with mixed solution of ethanol and acetic acid at a volume ratio of 8:1 for 8h, washed for several times, removed the template molecule and dried to obtain absolute molecular imprinted polymer. The absolute molecular imprinted polymer is dispersed in 1% chitosan acetic acid solution to prepare 20 g/L absolute molecular imprinted polymer solution. The 15-18 mu l solution is dropped in nano silver oxide/graphene modified electrode, followed by placing the infrared lamp to volatilize the solvent to obtain absolute molecular imprinted sensor. An INDEPENDENT CLAIM is also included for an absolute molecular imprinted sensor produced by the method.