▎ 摘　　要

NOVELTY - Preparing circulating tumor cell enrichment probe involves dissolving ammonium molybdate in nitric acid diluted solution, cracking at high temperature, centrifuging, washing, drying and synthesizing molybdenum oxide (MoO3) nano-rod, where mass volume ratio of ammonium molybdate and nitric acid is (0.5-1.5) g:(8-16) mL; at room temperature, dispersing MoO3nano-rod as template in water, adding pyrrole monomer, stirring to form solution A, then dissolving chloroauric acid (HAuCl4) and anhydrous ferric chloride in water to form solution B; where solution A in MoO3 nano rod, pyrrole monomer and water is (30-70) mg:(60-140) L:(30-60) mL; ratio of HAuCl4, anhydrous ferric chloride and water in solution B is (0.6-1.4) mL:(18-42) mg:(3-7) mL; gradually dropping solution B into solution A at normal temperature, continuously reacting. USE - Method for preparing circulating tumor cell enrichment probe. ADVANTAGE - The method enables to have high cell capture efficiency, excellent anti-interference performance and high selectivity, and avoids problems such as high cost and cumbersome operation. DETAILED DESCRIPTION - Preparing circulating tumor cell enrichment probe involves dissolving ammonium molybdate in nitric acid diluted solution, cracking at high temperature, centrifuging, washing, drying and synthesizing molybdenum oxide (MoO3) nano-rod, where mass volume ratio of ammonium molybdate and nitric acid is (0.5-1.5) g:(8-16) mL; at room temperature, dispersing MoO3nano-rod as template in water, adding pyrrole monomer, stirring to form solution A, then dissolving chloroauric acid (HAuCl4) and anhydrous ferric chloride in water to form solution B; where solution A in MoO3nano rod, pyrrole monomer and water is (30-70) mg:(60-140) L:(30-60) mL; ratio of HAuCl4, anhydrous ferric chloride and water in solution B is (0.6-1.4) mL:(18-42) mg:(3-7) mL; gradually dropping solution B into solution A at normal temperature, continuously reacting, under protection of nitrogen, slowly dripping concentrated ammonia water, further reacting, magnetic separation, washing, drying to obtain polypyrrole (PPy)-iron oxide (Fe3O4)-gold (Au) hollow nano-tube; dispersing PPy-Fe3O4-Au hollow nano-tube in 0.5-1.5 mg/mL buffer solution; adding suspension concentration as mercapto modified nucleic acid aptamer or specific antibody to suspension, ratio of two is (0.5-1.5mg/mL):(0.6-1.4 M), carrying out magnetic separation to obtain PPy-Fe3O4-Au-sulfydryl modified nucleic acid aptamer or specific antibody complex. An INDEPENDENT CLAIM is included for a method for using probe for constructing diagnostic sensor for circulating tumor cells, which involves: a. constructing electrochemiluminescence sensors to detect circulating tumor cells, based on PPy-Fe3O4-Au hollow tubular magnetic nano composite probe enriched target, graphene oxides (GO)-4-(4-hydroxymethyl-3-methoxyphenoxy)butyric acid (HMPB)-platinum (Pt) nano-composite immobilized electrochemical luminous body.