▎ 摘　　要

NOVELTY - A gold nanoparticles- beta -cyclodextrin-chitosan-graphene modified glassy carbon electrode preparation method involves preparing gold nanoparticles deposited solution by mixing chloroauric acid with buffer solution, and mixing carboxymethyl beta -cyclodextrin, amidogen- beta -cyclodextrin and beta -cyclodextrin. The modified gold nanoparticles electrodeposited layers are prepared by building a three-electrode system and subjecting to cyclic voltammetry. The cyclodextrin polymerization solution is prepared followed by preparing polymeric cyclodextrin modified electrical layer. USE - Method for preparing gold nanoparticles- beta -cyclodextrin-chitosan-graphene modified glassy carbon electrode for use in a glucose sensor for glucose detection. ADVANTAGE - The method enables preparing the gold nanoparticles- beta -cyclodextrin-chitosan-graphene modified glassy carbon electrode with improved stability of electrode resistance, linear range and reduced detection limit of electrode. DETAILED DESCRIPTION - A gold nanoparticles- beta -cyclodextrin-chitosan-graphene modified glassy carbon electrode preparation method involves preparing gold nanoparticles deposited solution by mixing 10 mg/ml chloroauric acid with 3-100 mu l potassium dihydrogen phosphate/potassium hydrogen phosphate buffer solution having concentration of 0.1 M/l and pH value of 8-12, mixing carboxymethyl beta -cyclodextrin, amidogen- beta -cyclodextrin and beta -cyclodextrin in the ratio of 0-1:0-1:0-1 and adding 5-100 mg mixture to the solution. The modified gold nanoparticles electrodeposited layers are prepared by building a three-electrode system comprising glassy carbon electrode as working electrode, molybdenum electrode as counter electrode, and silver/silver chloride electrode as reference electrode, subjecting system to cyclic voltammetry under scan range of -1. 4-0.6 V and scan rate 0.05-1 V/seconds, and storing modified glassy carbon electrodes in ultrapure water. The cyclodextrin polymerization solution is prepared by mixing carboxymethyl beta -cyclodextrin, amidogen- beta -cyclodextrin and beta -cyclodextrin in the ratio of 0-1:0-1:0-1, adding 0.1 M/l potassium dihydrogen phosphate/potassium hydrogen phosphate buffer solution with pH value of 4-6, stirring cyclodextrin solution with 2-10 mg/ml polymerization solution and mixing high-purity nitrogen gas to product for 10-20 minutes followed by preparing polymeric cyclodextrin modified electrical layer by obtaining modified glassy carbon electrode as working electrode, molybdenum electrode as counter electrode, and silver/silver chloride electrode as reference electrode, subjecting system to cyclic voltammetry under scan range of -1. 4-1 V and scan rate 0.05-0.5 V/seconds and subjecting product to electric polymerization.