▎ 摘　　要

NOVELTY - Biosensor comprises: a layer of graphene nanoplatelets, carbon nanotubes, or fullerene, a layer of at least one polymer on the layer of graphene nanoplatelets, carbon nanotubes, or fullerene, a layer of a ligand for the analyte on the polymer layer; and at least one sensor electrode. USE - The biosensor is useful for: detecting an analyte; and diagnosing a eye condition, where the eye condition is a full or partial thickness laceration or perforation to the anterior chamber of the eye, eye disease, mechanical or chemical eye injury, anterior scleral injury, corneal wound integrity, aqueous humor leaks, eye ulcer, infection, or surgical incisions (all claimed). ADVANTAGE - The biosensor utilized in the diagnosis of a eye condition provide a immediate result without sending the samples to a laboratory for further sampling or analysis; and detects the analytes rapidly, which provides reliable results. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are also included for: (1) a device comprising the biosensor and a detector connected to a data acquisition system; (2) a method (M1) of detecting an analyte in a sample, comprising contacting the biosensor with the sample and detecting the analyte with a detector; (3) a method (M2) of detecting an analyte in a sample, comprising contacting a sample with the biosensor, inserting the biosensor into or onto a device comprising a detector to measure change in electrical resistance, and a screen for visualizing the change in resistance, thus detecting an analyte in the sample; (4) a method (M3) of diagnosing an eye condition, comprising contacting the biosensor with a tear, tear film, aqueous layer of the tear film, or aqueous humor sample of a subject, detecting an amount of an analyte in the sample, and diagnosing an eye condition in the subject, where the concentration of the analyte in the sample is elevated as compared to a control sample or control standard; and (5) a method (M4) of diagnosing an eye condition in a subject, comprising detecting the amount of ascorbic acid in a tear film sample from the subject, and diagnosing an eye condition in the subject where the amount of ascorbic acid is elevated as compared to a control standard or control sample.