• 专利标题:   Preparing magnetic nano-material useful in extracting human whole blood genome DNA, comprises dispersing, coating and drying the zero-valent nano iron and graphene oxide.
  • 专利号:   CN111549023-A
  • 发明人:   CHEN Y, WANG X, XU R
  • 专利权人:   HANGZHOU MEDICAL COLLEGE
  • 国际专利分类:   C12N015/10, H01F001/00
  • 专利详细信息:   CN111549023-A 18 Aug 2020 C12N-015/10 202074 Pages: 8 Chinese
  • 申请详细信息:   CN111549023-A CN10320582 22 Apr 2020
  • 优先权号:   CN10320582

▎ 摘  要

NOVELTY - Preparing magnetic nano-material, comprises dispersing, coating and drying the zero-valent nano iron and graphene oxide. USE - The magnetic nano-material is useful in extracting human whole blood genome DNA (claimed). ADVANTAGE - The magnetic nano-material: does not affect subsequent PCR experiment. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is also included for using method of magnetic nano-material in extracting human whole blood genome DNA comprising (i) using 80-120 mu l of human whole blood sample and adding into 160-200 mu l lysis buffer, then placing in a 50-60 degrees C water bath for 8-12 minutes; (ii) adding 5-15 mg magnetic nanomaterials to the lysis solution obtained in the step (i), and then adding 200-300 mu l binding buffer, mixing by inversion for 0.5-2 minutes and then allowing to stand for 2-4 minutes; and (iii) using a magnet to magnetically separate the liquid after step (ii) to obtain a magnetic nano-material-DNA complex and supernatant, after removing the supernatant, washing the magnetic nanomaterial-DNA complex twice with 60-80% ethanol, adding 100-300 mu l Tris-EDTA buffer after drying, and eluting in 60-70 degrees C water bath for 8-12 minutes, and then centrifuging at 10000-14000 revolutions/minute (rpm) for 0.6-1.4 minutes, transferring the supernatant to a fresh Eppendorf tube, The Eppendorf tube contains the extracted human whole blood genomic DNA; the lysate described in step (i) contains 2 wt.% sodium dodecyl sulfate, 5 mu l RNase and 5 mu l protease; and the binding buffer described in step (ii) contains 15 wt.% PEG8000 and 1.25mol/l sodium chloride.