▎ 摘　　要

NOVELTY - Label-free detection of thrombin based on perylene derivative probe involves adding a to-be-detected sample to a detection system comprising oxidized graphene, perylene derivative (I) as a probe, a thrombin nucleic acid aptamer, a solvent and a buffer solution, detecting fluorescence intensity with a fluorescence spectrometer, and measuring the thrombin concentration through the fluorescence intensity. USE - Label-free detection of thrombin in to-be-detected sample e.g. aqueous solution, serum, saliva or cell lysate (all claimed). ADVANTAGE - The method enables simple, convenient, efficient and rapid label-free detection of thrombin in to-be-detected sample using economical materials under non-toxic, mild reaction conditions, with good selectivity, good stability and high sensitivity, and without requiring complex procedures and large instruments. In the label-free detection of thrombin, good linear response is in the range of 6 mU/mL, and the detection limit is as low as 0.082 mU/mL. The method solves the existing problems of needing fluorescent labels, poor stability, cumbersome steps, and high cost. DETAILED DESCRIPTION - Label-free detection of thrombin based on perylene derivative probe involves adding a to-be-detected sample to a detection system comprising oxidized graphene, perylene derivative of formula (I) as a probe, a thrombin nucleic acid aptamer, a solvent and a buffer solution, detecting fluorescence intensity with a fluorescence spectrometer, and measuring the thrombin concentration through the fluorescence intensity.