▎ 摘　　要

NOVELTY - Method for detecting target single nucleotide polymorphisms (SNP) involves (i) adding (a) a flap structure-specific degrading enzyme, (b) upstream probe (UP), (c) primary probe (P1), (d) secondary probe (P2) and (e) tertiary probe (P3), (ii) binding first probe (P1) and the upstream probe (UP) to the target nucleotide sequence comprising SNP, and cleaving 5'flap structure of the first probe using degrading enzyme, (iii) binding 5'flap of the primary probe to the 3'portion of the secondary probe (P2) and cleaving 5'flap structure of the secondary probe using the degrading enzyme, (iv) binding 5'flap cut to the 3'site of the tertiary probe and cleaving the 5'flap of the tertiary probe using the degrading enzyme, (v) combining the 5'flap cut in step (iv) with the secondary probe again, and (vi) repeating steps (iii)-(v) at least once to detect a signal indicating the presence of the target SNP. USE - The method is useful for detecting target SNP. ADVANTAGE - The method enables SNP detection without PCR reaction, and analyzes SNP in a rapid and cost-effective manner compared to traditional method at isothermal conditions. DETAILED DESCRIPTION - Method for detecting target single nucleotide polymorphisms (SNP) involves (i) adding (a) a flap structure-specific degrading enzyme, (b) upstream probe (UP) i.e. base pair sequence capable of complementarily binding to the 3'site of the target base pair sequence, (c) primary probe (P1) comprising a base pair sequence capable of complementarily binding to the 5'site of the target base sequence and a 5'flap capable of complementarily binding to the 3'site of the secondary probe, (d) secondary probe (P2) comprising a nucleotide sequence at the 3'site that can complementarily bind to the 5'flap generated from the primary probe and a 5'flap that can complementarily bind to the 3'site of the tertiary probe, and (e) tertiary probe (P3) comprising a nucleotide sequence at a 3'site that can complementarily bind to the 5'flap generated from the secondary probe and a 5'flap that can complementarily bind to the 3'site of the secondary probe, (ii) binding first probe (P1) and the upstream probe (UP) to the target nucleotide sequence comprising SNP, and cleaving 5'flap structure of the first probe using degrading enzyme, (iii) binding 5'flap of the primary probe to the 3'portion of the secondary probe (P2) and cleaving 5'flap structure of the secondary probe using the degrading enzyme, (iv) binding 5'flap cut to the 3'site of the tertiary probe and cleaving the 5'flap of the tertiary probe using the degrading enzyme, (v) combining the 5'flap cut in step (iv) with the secondary probe again, and (vi) repeating steps (iii)-(v) at least once to detect a signal indicating the presence of the target SNP. An INDEPENDENT CLAIM is included for SNP detection kit, which comprises a primary probe and an upstream probe that bind to a target base pair sequence comprising SNP, a secondary probe comprising a 3'portion to which the 5'flap of the cut primary probe can bind, a tertiary probe comprising a 3'portion to which the 5'flap of the cut secondary probe can bind, and FEN1 enzyme.