▎ 摘　　要

NOVELTY - Separation of graphene quantum dots involves mixing aqueous graphene quantum dots, hydrogen peroxide and ferric ion, performing Fenton reaction, dialyzing the reaction product using ultrapure water, to obtain aqueous solution of pure graphene quantum dot, mixing aqueous solution of graphene quantum dots with glycerol, performing gel electrophoresis separation of graphene quantum dots, cutting the gel, soaking the cut strips in ultrapure water, filtering to obtain graphene quantum dot fluorescent solution, and separating using atomic fluorescence spectrophotometer. USE - Separation of graphene quantum dot for use in biological and pharmaceutical applications. ADVANTAGE - The method enables simple and easy separation of graphene quantum dot. DETAILED DESCRIPTION - Separation of graphene quantum dots involves mixing aqueous graphene quantum dots, hydrogen peroxide as oxidant, and ferric ion as catalyst, performing Fenton reaction by irradiating ultraviolet radiation, dialyzing the reaction product using ultrapure water, removing unreacted hydrogen peroxide to obtain aqueous solution of pure graphene quantum dot, mixing aqueous solution of graphene quantum dots with glycerol, performing gel electrophoresis separation of graphene quantum dots at constant voltage, cutting the gel, soaking the cut strips in ultrapure water at room temperature for 3-24 hours, filtering the supernatant to obtain graphene quantum dot fluorescent solution, and separating using atomic fluorescence spectrophotometer. The gel is a polyacrylamide or agarose.