▎ 摘　　要

NOVELTY - A graphene oxide based on fluorescence quenching quantitatively analyzing method involves drawing function graph of graphene oxide concentration-fluorescence signal by preparing a series of graphene oxide solutions of the same volume and concentration, adding an equal volume of fluorescently modified ssDNA to the graphene oxide solution, detecting the fluorescence signal value of each mixed solution by using a fluorescence spectrophotometer, and plotting a relationship diagram in which the graphene oxide concentration is the abscissa and the fluorescence signal the ordinate followed by quantitative analyzing graphene oxide in the solution by adding an equal volume of the fluorescently modified ssDNA solution to the graphene oxide solution, detecting the fluorescent signal of the mixed solution by using a fluorescence spectrophotometer, and utilizing the above-mentioned graphene oxide concentration-fluorescence signal as a function of the relationship to calculate the concentration. USE - Method for quantitatively analyzing graphene oxide. ADVANTAGE - The method enables quantitatively analyzing graphene oxide in less time consumable, convenient and efficient manner. DETAILED DESCRIPTION - A graphene oxide based on fluorescence quenching quantitatively analyzing method involves drawing function graph of graphene oxide concentration-fluorescence signal by preparing a series of graphene oxide solutions of the same volume and concentration, adding an equal volume of fluorescently modified ssDNA to the graphene oxide solution, detecting the fluorescence signal value of each mixed solution by using a fluorescence spectrophotometer, and plotting a relationship diagram in which the graphene oxide concentration is the abscissa and the fluorescence signal the ordinate followed by quantitative analyzing graphene oxide in the solution by adding an equal volume of the fluorescently modified ssDNA solution to the graphene oxide solution to be tested, after thoroughly mixing, detecting the fluorescent signal of the mixed solution by using a fluorescence spectrophotometer, and utilizing the above-mentioned graphene oxide concentration-fluorescence signal as a function of the relationship to calculate the concentration of graphene oxide in the solution to be tested.