▎ 摘　　要

NOVELTY - Preparing a phenylboronic acid functionalized chitosan-modified graphene oxide nano material, comprises e.g. (a) dissolving graphene oxide in water, vortexing, ultrasonically mixing uniformly, and obtaining a concentration of aqueous solution of graphene oxide of 5-50 mg/ml, (b) dissolving chitosan oligosaccharide in an acidic organic solvent, vortexing and ultrasonically mixing uniformly to obtain a concentration of chitosan acid solution of 5-100 mg/ml, where the acidic organic solvent includes acetic acid, propionic acid or butyric acid, and (c) mixing the aqueous graphene oxide solution and the chitosan oligosaccharide solution at a volume ratio of 0.5:1-1.5:1, and then adding a glutaraldehyde solution, where the final mass percentage concentration of glutaraldehyde in the solution is 0.5-5%, reacting at 40-60 degrees C for 1-4 hours, after cooling to room temperature, sequentially washing with an organic solvent and water to obtain a graphene oxide/chitosan oligosaccharide suspension. USE - The method is useful for preparing phenylboronic acid functionalized chitosan-modified graphene oxide nano material. ADVANTAGE - The method improves the enrichment selectivity of glycoprotein and the enrichment capacity of glycoprotein. DETAILED DESCRIPTION - Preparing a phenylboronic acid functionalized chitosan-modified graphene oxide nano material, comprises: (a) dissolving graphene oxide in water, vortexing, ultrasonically mixing uniformly, and obtaining a concentration of aqueous solution of graphene oxide of 5-50 mg/ml, (b) dissolving chitosan oligosaccharide in an acidic organic solvent, vortexing and ultrasonically mixing uniformly to obtain a concentration of chitosan acid solution of 5-100 mg/ml, where the acidic organic solvent includes acetic acid, propionic acid or butyric acid, (c) mixing the aqueous graphene oxide solution and the chitosan oligosaccharide solution at a volume ratio of 0.5:1-1.5:1, and then adding a glutaraldehyde solution, where the final mass percentage concentration of glutaraldehyde in the solution is 0.5-5%, reacting at 40-60 degrees C for 1-4 hours, after cooling to room temperature, sequentially washing with an organic solvent and water to obtain a graphene oxide/chitosan oligosaccharide suspension, and (d) dissolving phenylboronic acid in an organic solvent, vortexing and mixing uniformly, adding the graphene oxide/chitooligosaccharide suspension, reacting at room temperature for 12-48 hours, and sequentially washing with an organic solvent and water to obtain hydrophilic phenylboronic acid functionalized chitosan modified graphene oxide nanomaterial, where the concentration of the phenylboronic acid in the solution is 1-100 mg/ml, and the organic solvent is methanol or ethanol.