▎ 摘　　要

NOVELTY - Zeolite imidazolate framework-67/reduced graphene oxide (ZIF-67/rGO) nanozyme having a three-dimensional graded structure having daisy shape, is new. USE - The ZIF-67/rGO nanozyme is useful for detecting hydrogen peroxide content (claimed). ADVANTAGE - The ZIF-67/rGO nano-enzyme solves the problem that the existing detection method has high detection lower limit and long detection response time. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are also included for: (1) preparing ZIF-67/rGO nano-enzyme; (2) rapid detection of hydrogen peroxide content, comprising using ZIF-67/rGO nanozyme to catalyze the reaction of hydrogen peroxide with the substrate to generate a blue oxidized substrate, using spectrophotometry to measure the absorbance of the reaction solution to calculate the content of hydrogen peroxide, (i) preparing standard curve, (i.i) preparing 0.5-2 mg/ml 3,3',5,5'-tetramethylbenzidine (TMB) aqueous solution and 0.1-1 mg/ml ZIF-67/rGO nanoenzyme aqueous solution with a volume ratio of 1:1, taking TMB aqueous solution, buffer solution, deionized water and ZIF-67/rGO nanoenzyme aqueous solution with a volume ratio of 50-400:100-600:100-1000:50-300 respectively, and mixing together as a substrate solution, and configuring multiple same substrate solutions, (i.ii) adding hydrogen peroxide standard solutions with concentrations of 1 mu M, 2.5 mu M, 5 mu M, 7.5 mu M, 10 mu M, 25 mu M, 50 mu M, 75 mu M, 100 mu M, 150 mu M respectively, where volume ratio of hydrogen peroxide and substrate solution is 100-300:1200-1400, and mixing well after constant volume, and reacting, (i.iii) testing the absorption spectrum of the mixed solution in the wavelength range of 300-1000 nm after the reaction, and recording the absorbance values A1, A2, A3, A4, A5, A6, A7, A8, A9, A10 corresponding to the 652nm wavelength respectively, taking log value of the concentration of multiple aqueous hydrogen peroxide solutions, taking absorbance value as the ordinate, and plotting the log value of the hydrogen peroxide concentration on the abscissa, i.e., standard curve of hydrogen peroxide, (ii) determining content of hydrogen peroxide, (ii.i) preparing 0.5-2 mg/ml TMB aqueous solution and 0.1-1 mg/ml ZIF-67/rGO nanoenzyme aqueous solution with a volume ratio of 1:1, taking TMB aqueous solution, buffer solution, deionized water and ZIF-67/rGO nanoenzyme aqueous solution with a volume ratio of 50-400:100-600:100-1000:50-300 respectively, and mixing them together as a substrate solution, (ii.ii) adding test solution of hydrogen peroxide, where volume ratio of hydrogen peroxide to the substrate solution is 100-300:1200-1400, then mixing and reacting, (ii.iii) testing absorption spectrum of the mixed solution in the wavelength range of 300-1000 nm after the reaction, recording absorbance value corresponding to the wavelength of 652 nm, and using standard curve of hydrogen peroxide obtained in step (i.iii) and calculating the content of hydrogen peroxide in the hydrogen peroxide test solution; and (3) biosensor platform for detecting hydrogen peroxide, comprising wide-band detection light source system, narrow-band filter system, optical collimation system, biosensor, translation device and optical detector arranged in order from top to bottom. The wide-band detection light source system is used to perform a full-spectrum absorption test on the biosensor. The narrowband filter system is used to perform a specific wavelength light absorption test on the biosensor. The optical collimation system is used to converge and illuminate the divergent light source on the biosensor. The biosensor is installed above the translation device and biosensor comprises detection substrate. The detection substrate comprises a substrate solution and ZIF-67/rGO nanozyme. The optical detector is used to convert the collected optical signal into an electrical signal. DESCRIPTION OF DRAWING(S) - The drawing shows a ZIF-67/rGO nanoenzyme transmission electron microscope image.