• 专利标题:   Detecting virus particles, preferably infectious virus particles in sample, or preventing spread of pandemic viral infections, preferably severe acute respiratory syndrome-coronavirus, by incubating sample with virus-binding molecule bound to solid phase, and detecting binding of virus particles.
  • 专利号:   WO2021245170-A1, CA3179389-A1, EP4162274-A1, US2023213517-A1
  • 发明人:   LOIBNER H, POGLITSCH M
  • 专利权人:   COVIRABIO GMBH, COVIRABIO GMBH, COVIRABIO GMBH
  • 国际专利分类:   G01N033/569, C12Q001/6888, C12Q001/70
  • 专利详细信息:   WO2021245170-A1 09 Dec 2021 202104 Pages: 36 English
  • 申请详细信息:   WO2021245170-A1 WOEP064857 02 Jun 2021
  • 优先权号:   AT050486, CA3179389

▎ 摘  要

NOVELTY - Method for detecting virus particles in a sample involves (a) incubating the sample with at least one virus-binding molecule bound to a solid phase, and (b) detecting binding of virus particles to at least one virus-binding molecule bound to the solid phase. USE - The method is useful for detecting virus particles in a sample. The virus particles are infectious virus particles (all claimed). The method is also used for preventing spread of pandemic viral infections, preferably SARS-CoV-2. ADVANTAGE - The method rapidly detects virus particles in a sample. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is included for a kit, which comprises (i) a manual, one or more solvents, one or more buffers, and/or one or more solid phases and/or one or more enzymes and/or one or more antibodies and/or one or more primers and/or one or more enzyme substrates and/or one or more inactivated virus or pseudo virus preparations or (ii) a solid phase, preferably a plate such as a 96-well plate or beads such as agarose beads or a solid phase having a graphene surface or a solid phase having a semiconducting surface, a virus entry receptor for the virus particles bound to the solid phase, where the virus entry receptor bound to the solid phase is enzymatically inactive, preferably a soluble virus entry receptor for the virus particles, where the soluble virus entry receptor is enzymatically active, at least one substrate for the enzymatic activity of the soluble virus entry receptor, at least one washing solution, and at least one inactivated virus or pseudo virus preparations, where the virus entry receptor bound to the solid phase is enzymatically inactive angiotensin-converting enzyme 2 (ACE2), and the soluble virus entry receptor is soluble enzymatically active ACE2, where the virus particles are severe acute respiratory syndrome (SARS)-coronavirus (CoV) particles, SARS-CoV-2 particles or HCoV-NL63 particles, preferably SARS-CoV-2 particles.