▎ 摘　　要

NOVELTY - Gene detection chip comprises upper substrate, an intermediate layer and a lower substrate that are bonded to each other. The area of the lower substrate that is attached to the sample injection channel is covered with graphene oxide and is attached to the microcavity. Poly-lysine is arranged on the combined area. The side of the intermediate layer in contact with the lower substrate is engraved with a sampling flow channel and a microcavity communicating with the sampling flow channel, and a sampling flow is also connected to the sampling flow channel. The sample injection channel is provided with a sample inlet, and the sample outlet channel is provided with a sample outlet. The side of the upper substrate in contact with the intermediate layer is engraved with a microcavity control channel and a sample output control channel. The microcavity control flow channel is provided with a microcavity control valve. USE - The gene chip is useful for detection of gene (claimed). ADVANTAGE - The gene chip detects gene simply and cost-effectively, and has stable performance. DETAILED DESCRIPTION - Gene detection chip comprises upper substrate, an intermediate layer and a lower substrate that are bonded to each other. The area of the lower substrate that is attached to the sample injection channel is covered with graphene oxide and is attached to the microcavity. Poly-lysine is arranged on the combined area. The side of the intermediate layer in contact with the lower substrate is engraved with a sampling flow channel and a microcavity communicating with the sampling flow channel, and a sampling flow is also connected to the sampling flow channel. The sample injection channel is provided with a sample inlet, and the sample outlet channel is provided with a sample outlet. The side of the upper substrate in contact with the intermediate layer is engraved with a microcavity control channel and a sample output control channel. The microcavity control flow channel is provided with a microcavity control valve, the sample discharge control flow channel is provided with a sample discharge control valve, and the microcavity control valve is located above the sample injection flow channel near the entrance of the microcavity. The sample control valve is located above-mentioned sample flow channel. An INDEPENDENT CLAIM is included for detection method of the gene detection chip, which involves injecting liquid into the microcavity control flow channel and sample discharge control flow channel in the upper substrate, thus opening and closing of the microcavity control valve and the sampling control valve is controlled by nitrogen later, injecting the synthesized target gene with fluorophore into the injection port, where the microcavity control valve on the upper substrate is in the closed state, and the sample output control valve is in the open state, target gene of the fluorophore is blown to the sample outlet through the injection channel and the sample outlet channel, the positive charge on the surface of the graphene oxide on the lower substrate and the negative charge on the surface of the target gene are mutually adsorbed by the positive and negative charges, the target gene with a fluorescent group is laid on the injection channel on the lower substrate, and the fluorescent group is quenched by graphene oxide, after incubating the target gene with fluorescent group and the graphene oxide arranged on the lower substrate in advance for a period of time, sucking excess unadsorbed target gene from the sample outlet, injecting buffer into the sample inlet, aspirating the buffer from the sample outlet, repeating for several times, thoroughly washing excess unadsorbed target gene, injecting the gene from the injection port, closing the micro-cavity control valve and the sample discharge control valve, using nitrogen to blow the gene into the sample flow channel, wher the gene is same as the original one, combining target gene with fluorescent group into double-stranded DNA, adsorbing graphene oxide with single-stranded, not double-stranded, suspending double-stranded DNA in the injection channel, opening microcavity control valve, blowing nitrogen with double-stranded DNA into the microcavity, arranging polylysine on the microcavity on the lower substrate that adsorbs the double-stranded DNA, restoring the fluorescence, and using lower substrate for fluorescence detection.