▎ 摘　　要

NOVELTY - Constructing and analyzing electrochemical aptamer sensor, comprises using carbon ionic liquid electrode (CILE) as a base electrode, forming a reduced graphene oxide film on the surface by the electro-reduction method, modifying reduced graphene oxide with carboxylated multi-walled carbon nanotubes, loading the carboxylated multi-walled carbon nanotubes with gold nanoparticles, using gold nanoparticles for self-assembly of thiolated aptamer probes, and using thiolated aptamer probe to specifically recognize heavy metal lead ions. USE - The sensor is useful for lead ion detection (claimed). ADVANTAGE - The method: induces thiolated aptamer probes to form a G-quadruplex conformation; facilitates the intercalation of the electrochemical indicator hemin (Hemin); utilizes the electrochemical methods to detect Hemin's electrical signal, and strength of the electrical signal related to the concentration of lead ions; and realizes the quantitative analysis of lead ions. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are also included for: (1) constructing and analyzing of electrochemical aptamer sensor device, comprises (a) coating 10 mu l 0.5 mg/ml graphene oxide dispersion evenly on the surface of CILE, drying at room temperature and put it into 10 mmol/l of pH 7.7, using tris-hydrochloride buffer solution for electrochemical reduction at a reduction potential of -1.3 V for 200 seconds, washing rGO/CILE with ultrapure water, and naturally drying, (ii) adding COOH-MWCNT to the dispersant N'N-dimethylformamide for uniform ultrasonic dispersion to obtain a COOH-MWCNT dispersion, taking 8 mu l 2 mg/ml dispersion, uniformly coating COOH-MWCNT/rGO/CILE, and drying the rGO/CILE surface at room temperature, (iii) placing the COOH-MWCNT/rGO/CILE into 2 mmol/l chloroauric acid solution for electrochemical deposition with a deposition potential of -0.3 V for 100 seconds, washing with ultrapure water and drying to obtain Au/COOH-MWCNT/rGO/CILE, and (iv) placing the Au/COOH-MWCNT/rGO/CILE into 1 mu mol/l thiolated aptamer probe and self-assemble at 4 degrees C for 12 hours, forming Au-S bond, fixing thiolated aptamer probe on the modified electrode, taking out, rinsing with ultrapure water to remove the unassembled aptamer chain adsorbed on the electrode surface, dripping 30 mu l 1 mmol/l thioglycolic acid, reacting at 25 degrees C for 0.5 hours, sealing the free binding sites on the electrode surface, washing with ultrapure water, and naturally drying; (2) constructing and analyzing electrochemical aptamer sensor, comprising reacting sulfhydryl aptamer probe with different concentrations of lead ions for 40-60 minutes; and (3) constructing and analyzing electrochemical aptamer sensor device, comprising binding G-quadruplex conformation induced by lead ions with Hemin for 10-60 minutes, and the concentration of Hemin dispersion is 1-10 mu mol/l.