• 专利标题:   New probe useful for detecting lead ion concentration comprises probe A combined with graphene quantum dot and probe B bound with gold nanoparticle.
  • 专利号:   CN108254343-A
  • 发明人:   LUO D, NIU X
  • 专利权人:   UNIV SOUTHERN SCI TECHNOLOGY
  • 国际专利分类:   G01N021/64
  • 专利详细信息:   CN108254343-A 06 Jul 2018 G01N-021/64 201860 Pages: 10 Chinese
  • 申请详细信息:   CN108254343-A CN11487178 29 Dec 2017
  • 优先权号:   CN11487178

▎ 摘  要

NOVELTY - A probe comprising probe A combined with a graphene quantum dot and a probe B bound with a gold nanoparticle, is new. The probe A has a 33 base pair sequence (SEQ ID NO: 1) fully defined in the specification and probe B comprises SEQ ID NO: 2. USE - The probe is useful for detecting the lead ion concentration (claimed). ADVANTAGE - The probe is prepared by a simple process. DETAILED DESCRIPTION - A detection probe comprising probe A combined with a graphene quantum dot and a probe B bound with a gold nanoparticle, is new. The probe A has a 33 base pair sequence (SEQ ID NO: 1) fully defined in the specification and probe B comprises SEQ ID NO: 2. cgataactcactata/gaggaagagatg (SEQ ID NO: 2). INDEPENDENT CLAIMS are included for the following: (1) method of preparing a detection probe involves (a) mixing the gold nanoparticle with the probe B having a thiol group, adding sodium chloride and sodium hydrogen phosphate and centrifuging the contents for removing the supernatant and (b) mixing the graphene quantum dots with the probe A having an amino group, adding the 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) solution, mixing the contents and leaving the mixed probe; and (2) method for detecting the lead ion concentration involves placing the probe A and probe B under the excitation light having a wavelength of 370-420 nm, preferably 380-410 nm and adding lead ions for performing spectral signal detection.