▎ 摘　　要

NOVELTY - Preparing graphene oxide-based enzyme-free glucose sensor by electron beam irradiation method comprises e.g. (i) dissolving potassium persulfate and phosphorus pentoxide in concentrated sulfuric acid, adding natural graphite, adding potassium permanganate, adding 30% hydrogen peroxide to obtain solid graphene oxide, and (ii) dispersing graphene oxide in deionized water, dissolving soluble silver salt in suspension, adding aniline into first mixed solution, adding perchloric acid solution into second mixed solution, placing third mixed solution in a sealed reaction vessel, washing and drying. USE - The method is useful for preparing graphene oxide-based enzyme-free glucose sensor by electron beam irradiation method (claimed). ADVANTAGE - The method is simple, has low cost and good biocompatibility; and does not involve toxic substances, and is environmentally friendly. DETAILED DESCRIPTION - Preparing graphene oxide-based enzyme-free glucose sensor by electron beam irradiation method comprises (i) dissolving potassium persulfate and phosphorus pentoxide in concentrated sulfuric acid and heating at 80 degrees C, adding natural graphite, incubating at 80 degrees C for 4.5 hours and then cooling to room temperature, diluting with deionized water and placing overnight, filtering, washing, drying resulting residue in a vacuum oven at 60 degrees C, dissolving dried residue in concentrated sulfuric acid, adding potassium permanganate under ice-cooling, stirring at 35 degrees C for 2 hours at constant temperature, then diluting with deionized water, adding 30% hydrogen peroxide, filtering, washing, drying washed product in vacuum at 60 degrees C to obtain solid graphene oxide, and (ii) (a) dispersing graphene oxide in deionized water, placing in ultrasonic oscillator, performing ultrasonic dispersion for 5-15 minutes, mixing to obtain suspension of graphene oxide at concentration of 0.5-2 g/l, (b) dissolving soluble silver salt in suspension and then placing in the ultrasonic oscillator, performing ultrasonic dispersion for 5-8 minutes, mixing mixture to obtain first mixed solution, where mass ratio between silver salt and graphene oxide is 1:1-1:5, (c) adding 150-300 mu l aniline into first mixed solution, placing in magnetic stirrer for 20-30 minutes, mixing to obtain second mixed solution, (d) adding 4-15 ml perchloric acid solution into second mixed solution, stirring, mixing them uniformly to obtain third mixed solution, (e) placing third mixed solution in a sealed reaction vessel and irradiating it under irradiation of an electron beam generated by an electron accelerator of 2.5 MeV and 40 mA, where irradiation dose is 150-300 KGy to obtain product, (f) washing irradiated product with ethanol and distilled water, and then centrifugating with high-speed centrifuge, separating, repeating many times to remove unreacted ions to obtain separated solid matter, and (g) drying separated solid in vacuum oven at 50-80 degrees C for 8-15 hours to obtain final product.