▎ 摘　　要

NOVELTY - Detecting microbial antigen in biological fluid comprises (a) preparing dual confirmatory sensor based on electrochemical and enzyme-linked immunosorbent technologies (ELISA) techniques for detection of microbial antigen in biological fluid; (b) measuring electrochemical response to provide sensing of microbial antigen in biological fluid using modified screen printed electrode; (c) detecting microbial antigen directly from biological fluid based on presence of immunoglobulin G in ELISA well equipped with modified screen printed electrode, where the preparation of dual confirmatory sensor based on electrochemical and ELISA techniques for detection of microbial antigen in biological fluids comprises: mixing 1-5% chitosan Nafion(RTM: Sulfonated tetrafluoroethylene based fluoropolymer-copolymer) with graphene quantum dots, gold nanoparticles, composite of graphene quantum dots or composite of gold nanoparticles, sonicating mixture, and screen printing electrode with mixture. USE - The method is useful for detecting microbial antigen in a biological fluid. ADVANTAGE - The method utilizes sensor, which is economical, reliable, and specific, and sensitive detection of microbes to SARS, and H1N1 for normal human and in disease condition. DETAILED DESCRIPTION - Detecting microbial antigen in a biological fluid comprises (a) preparing the dual confirmatory sensor based on electrochemical and enzyme-linked immunosorbent technologies (ELISA) techniques for detection of microbial antigen in biological fluid; (b) measuring electrochemical response to provide sensing of microbial antigen in biological fluid using modified screen printed electrode; (c) detecting microbial antigen directly from biological fluid based on presence of immunoglobulin G (IgG) in ELISA well equipped with modified screen printed electrode, where the preparation of dual confirmatory sensor based on electrochemical and ELISA techniques for detection of a microbial antigen in biological fluids comprises: mixing 1-5% chitosan Nafion(RTM: Sulfonated tetrafluoroethylene based fluoropolymer-copolymer) with graphene quantum dots, gold nanoparticles, composite of graphene quantum dots or composite of gold nanoparticles to obtain mixture, sonicating mixture, screen printing electrode with mixture, leaving the electrode for drying, and washing electrode with phosphate buffer saline (PBS). An INDEPENDENT CLAIM is also included for dual confirmatory sensor based on ELISA techniques for detection of a microbial antigen in biological fluid, comprising screen printed electrode modified (modified SPE) with graphene quantum dots or gold nanoparticles, composite of graphene quantum dots or composite of gold with 1-5% chitosan Nafion(RTM: Sulfonated tetrafluoroethylene based fluoropolymer-copolymer), where the dual confirmatory sensor is configured to: measures electrochemical response of modified SPE in the presence of different concentration of the microbial antigen by cyclic voltammetry, differential pulse voltammetry, and impedance spectroscopy, and detect the microbial antigen directly from the biological fluid based on presence of IgG.