▎ 摘　　要

NOVELTY - Preparing graphene carrier involves dissolving aniline-4- beta -ethyl sulfonyl sulfate-2-sulfonic acid (SESA) into distilled water at 30-50 degrees C. Sodium carbonate (1mol/L) is added into the obtained solution for regulating pH value to neutral. Graphene oxide is added into the solution. Sodium hydroxide (0.5mol/L) is added to the solution for adjusting the pH value 9.0-14.0. Etherification reaction is performed to the obtained solution. The obtained solution is washed subsequently with distilled water to obtain ethyl-amino-benzenesulfonyl graphene oxide (ABSE-GO), after completion of reaction. USE - Method for preparing graphene carrier used in immobilization of protein, where protein comprises cellulose, penicillin G acylase or bovine serum albumin (claimed). ADVANTAGE - The method enables to prepare graphene carrier, which is fast and has research value. DETAILED DESCRIPTION - Preparing graphene carrier involves dissolving aniline-4- beta -ethyl sulfonyl sulfate-2-sulfonic acid (SESA) into distilled water at 30-50 degrees C. Sodium carbonate (1mol/L) is added into the obtained solution for regulating pH value to neutral. Graphene oxide is added into the solution. Sodium hydroxide (0.5mol/L) is added to the solution for adjusting the pH value 9.0-14.0. Etherification reaction is performed to the obtained solution. The obtained solution is washed subsequently with distilled water to obtain ethyl-amino-benzenesulfonyl graphene oxide (ABSE-GO), after completion of reaction. Diazotization reaction is performed by adding distilled water, hydrochloric acid solution and sodium nitrite into ABSE-GO. The obtained material is washed subsequently with hydrochloric acid solution. The obtained material is vacuum-dried to obtain graphene carrier. An INDEPENDENT CLAIM is included for a method for applying graphene carrier in immobilized protein, which involves: (A) diluting graphene oxide (25-50mg) in centrifugal tube, adding protein solution (0.2-1ml) and buffer solution (pH 3.0-12.0), making total reaction volume of 1ml, performing centrifugation for 1-60minutes; (B) removing centrifugal supernatant and adding 1ml of buffer liquid, mixing uniformly, after centrifugation, removing centrifugal supernatant and adding 1ml of buffer liquid, mixing uniformly, after centrifugation; and (C) removing all supernatant, using Brandford method to determine protein content in supernatant, calculating immobilized yield by using 3,5-dinitrosalicylic acid chromogenic assay method or p-dimethylaminobenzaldehyde method and measuring enzyme activity of immobilized enzyme and then calculating immobilization efficiency.