▎ 摘　　要

NOVELTY - An enzyme electrode producing method involves adding 1-amino-pyrene and reducing graphene oxide in anhydrous ethanol, dispersing, washing and centrifuging. The material is added with phosphate-buffered saline and dispersed. The dispersion material is added with glutaraldehyde solution and allowed to react for 10-60 minutes, followed by adding tyrosinase solution in solution system, shaking to obtain enzyme solution, applying enzyme solution in screen-printed electrode after activation treatment to obtain the finished product. USE - Method for producing enzyme electrode that is utilized in determination of phenol, and analytical chemistry and environment monitoring field. ADVANTAGE - The method enables producing enzyme electrode with high sensitivity and good selectivity, in simple, convenient, fast and cost effective manner. DETAILED DESCRIPTION - An enzyme electrode producing method involves adding 0.05-0.3 mg 1-amino-pyrene and 0.5-3 mg reducing graphene oxide in 1 ml anhydrous ethanol, dispersing ultrasonically for 0.5-8 hours, allowing to stand for overnight, washing and centrifuging under drying at 70 degrees C to obtain reducing graphene oxide/1-amino-pyrene material. The reducing graphene oxide/1-amino-pyrene material is added with phosphate-buffered saline buffer solution having pH of 6.0-7.0 and dispersed ultrasonically for 15-60 minutes to obtain reducing graphene oxide/1-amino-pyrene dispersion material having dispersion concentration of 1-4 mg/ml. The 0.2 ml graphene oxide/1-amino-pyrene dispersion material is taken, added with 0.2 ml 2-10 mass% glutaraldehyde solution, shaken well and allowed to react for 10-60 minutes to obtain solution system, followed by adding 0.2 ml tyrosinase solution in solution system, shaking, allowing to react for 10-60 minutes to obtain reducing graphene oxide/1-amino-pyrene-glutaraldehyde-tyrosinase enzyme solution, applying 10-30 mu l reducing graphene oxide/1-amino-pyrene-glutaraldehyde-tyrosinase enzyme solution in screen-printed electrode after activation treatment to obtain the finished product, where the tyrosinase solution is obtained by dissolving tyrosinase in phosphate-buffered saline buffer solution having pH of 6.0-7.0 to obtain phosphate-buffered saline buffer solution. An INDEPENDENT CLAIM is also included for an enzyme electrode prepared by the method.